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	Provedor de dados Scientia Agricola (1) Electron. J. Biotechnol. (1) Autor Mliki,Ahmed (2) Bouamama,Badra (1) Buhner-Zaharieva,Tatiana (1) Ghorbel,Abdelwahed (1) Gogorcena,Yolanda (1) Gouta,Hassouna (1) Jardak,Rahma (1) Ksia,Elhem (1) Salem,Asma Ben (1) Mais... Palavra-chave Forced anthers (1) Genetic diversity (1) Genetic resources (1) Long-term maintenance (1) Microsatellites (1) Molecular characterization (1) Prunus dulcis Mill (1) Somatic embryos (1) Vitis vinifera (1) Mais... Tipo do documento Info:eu-repo/semantics/article (1) Journal article (1) Ano 2012 (1) 2009 (1) País Brazil (1) Chile (1) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Preservation of endangered Tunisian grapevine cultivars using embryogenic cultures Electron. J. Biotechnol. Bouamama,Badra; Jardak,Rahma; Salem,Asma Ben; Ghorbel,Abdelwahed; Mliki,Ahmed. The preservation of embryogenic lines derived from several endangered local grapevine cultivars was studied. Embryogenic calluses were obtained from immature anthers of eight cultivars, sampled on both fruity-cuttings and field grown vines. Anthers at the 'separated flower' stage, derived from fruity-cuttings, resulted in an increased induction of somatic embryogenesis, compared to those derived from the field. Pro-embryogenic calluses were induced on Chée and Pool (1987) basal medium, supplemented with 9 µM of 2,4-dichlorophenoxyacetic acid (2,4-D) and 11.35 µM of thidiazuron (TDZ) under dark conditions. Different anther zones (filament, abaxial, adaxial, lateral zones and entire anthers) were involved in somatic embryogenesis induction. The percentages... Tipo: Journal article Palavras-chave: Forced anthers; Long-term maintenance; Somatic embryos; Vitis vinifera. Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582009000200006 Development of an SSR-based identification key for Tunisian local almonds Scientia Agricola Gouta,Hassouna; Ksia,Elhem; Buhner-Zaharieva,Tatiana; Mliki,Ahmed; Gogorcena,Yolanda. Ten simple sequence repeat (SSR) loci were used to study polymorphism in 54 almond genotypes. All genotypes used in this study originated from almond-growing areas in Tunisia with different climatic conditions ranging from the sub-humid to the arid and are preserved in the national collection at Sidi Bouzid. Using ten SSR, 130 alleles and 250 genotypes were revealed. In order to develop an identification key for each accession, the data were analysed separately for each microsatellite marker. The most polymorphic microsatellite (CPDCT042) was used as a first marker. Two microsatellite loci (CPDCT042 and CPDCT025) were sufficient to discriminate among all accessions studied. Neighbour-joining clustering and principal coordinate analysis were performed to... Tipo: Info:eu-repo/semantics/article Palavras-chave: Prunus dulcis Mill; Genetic diversity; Genetic resources; Microsatellites; Molecular characterization. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162012000200004 Registros recuperados: 2 Primeira ... 1 ... Última

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