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	Provedor de dados 69 (2) 58 (1) Autor Moeini,Hassan (3) Abdullah,Mohd Puad (1) B. Ariff,Arbakariya (1) Dezfouli,Maryam Ghandizadeh (1) Dinarvand,Mojdeh (1) Eissazadeh,Samira (1) Heidary,Somayyeh (1) Masomian,Malihe (1) Mousavi,Seyed Sadegh (1) Mustafa,Shuhaimi (1) Nahavandi,Reza (1) Nahvi,Iraj (1) Nelofer,Rubina (1) Tavassoli,Manoochehr (1) Mais... Palavra-chave Artificial neural network (1) Aspergillus niger ATCC 20611 (1) BOD (1) Beta-galactosidase (1) Candida versatilis (1) Culture optimization (1) Human epidermal growth factor (1) Inulinase (1) Kluyveromyces lactis (1) Kluyveromyces marxianus (1) Mixed culture (1) Pichia pastoris (1) SCP (1) Whey (1) Mais... Tipo do documento Journal article (2) Info:eu-repo/semantics/article (1) Ano 2017 (1) 2012 (1) 2004 (1) País Chile (2) Brazil (1) Idioma Inglês (2) Espanhol (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Production of recombinant human epidermal growth factor in Pichia pastoris 58 Eissazadeh,Samira; Moeini,Hassan; Dezfouli,Maryam Ghandizadeh; Heidary,Somayyeh; Nelofer,Rubina; Abdullah,Mohd Puad. Abstract This study was carried out to express human epidermal growth factor (hEGF) in Pichia pastoris GS115. For this aim, the hEGF gene was cloned into the pPIC9K expression vector, and then integrated into P. pastoris by electroporation. ELISA-based assay showed that the amount of hEGF secreted into the medium can be affected by the fermentation conditions especially by culture medium, pH and temperature. The best medium for the optimal hEGF production was BMMY buffered at a pH range of 6.0 and 7.0. The highest amount of hEGF with an average yield of 2.27 µg/mL was obtained through an induction of the culture with 0.5% (v/v) methanol for 60 h. The artificial neural network (ANN) analysis revealed that changes in both pH and temperature significantly... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pichia pastoris; Human epidermal growth factor; Artificial neural network. Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000200286 Improvement of SCP production and BOD removal of whey with mixed yeast culture 69 Moeini,Hassan; Nahvi,Iraj; Tavassoli,Manoochehr. This research emphasizes on single cell protein (SCP) production and Biochemical Oxygen Demand (BOD) removal from whey with mixed yeast culture. For this purpose, 11 yeast strains were isolated from dairy products (M1-M11) and the strains were identified by morphological and physiological properties. These yeast strains were tested for their ability to reduce the BOD and to produce SCP from whey. Among these strains, K. lactis (M2) had the most SCP production from whey with the yield of 11.79 g/l. Ammonium sulphate as nitrogen source had an increasing effect on biomass yield. The mixed culture of the isolated yeast strains with Saccharomyces cerevisiae was used in order to increase the biomass yield and BOD removal. The highest biomass yield (22.38 g/l)... Tipo: Journal article Palavras-chave: Beta-galactosidase; BOD; Candida versatilis; Kluyveromyces lactis; Kluyveromyces marxianus; Mixed culture; SCP; Whey. Ano: 2004 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582004000300006 Effect of extrinsic and intrinsic parameters on inulinase production by Aspergillus niger ATCC 20611 69 Dinarvand,Mojdeh; B. Ariff,Arbakariya; Moeini,Hassan; Masomian,Malihe; Mousavi,Seyed Sadegh; Nahavandi,Reza; Mustafa,Shuhaimi. Background: Inulinase is a versatile enzyme from glycoside hydrolase family which targets the β-2, 1 linkage of fructopolymers. In the present study, the effect of medium composition and culture conditions on inulinase production by Aspergillus niger ATCC 20611 was investigated in shake-flasks. Results: The highest extracellular inulinase (3199 U/ ml) was obtained in the presence of 25% (w/v) sucrose, 0.5% (w/v) meat extract, 1.5% (w/v) NaNO3 and 2.5 mM (v/v) Zn2+, at initial pH of 6.5, temperature 35ºC and 6% (v/v) of spores suspension in the agitation speed of 100 rpm. Surfactants showed an inhibitory effect on enzyme production. The optimum temperature for inulinase activity was found to be 50ºC. TLC analysis showed the presence of... Tipo: Journal article Palavras-chave: Aspergillus niger ATCC 20611; Culture optimization; Inulinase. Ano: 2012 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582012000400005 Registros recuperados: 3 Primeira ... 1 ... Última

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