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Quantitative Study of the Myenteric Plexus of the Descending Colon of Young Rats Subjected to Intense Protein Deficiency International Journal of Morphology
Araújo,Eduardo José de Almeida; Sant'Ana,Débora de Mello Gonçales; Molinari,Sonia Lucy; Miranda Neto,Marcílio Hübner de.
We studied the effects of a severely hypoproteic diet on the quantitative aspects of the myenteric plexus of the descending colon of young rats. Eighteen rats were divided into two groups, one of them being fed with a chow having 26% protein (control) and the other with a chow having 4% protein, balanced for minerals and vitamins, during 12 weeks. The whole-mounts of the descending colon had their myenteric neurons stained either with Giemsa or NADPH diaphorase. The rats from the experimental group had deficits of body weight (54.23%) and area of the descending colon (48.14%); additionally, we observed that there was no alteration in the total number of neurons of the colon, but a decrease in the number of NADPH-diaphorase positive neurons (37.80%). The...
Tipo: Journal article Palavras-chave: Myenteric plexus; Protein-energy; Malnutrition (PEM); Descending colon.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000500013
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Morphological and Quantitative Analyses of the NADH-d Myenteric Neurons of the Jejunum of Rats Submitted to Experimental Chronic Alcoholism International Journal of Morphology
Pereira,Marli Aparecida dos Santos; Arantes,Márcia Alessandra; Molinari,Sonia Lucy; Zanoni,Jacqueline Nelisis; Miranda-Neto,Marcílio Hubner de.
The objective of our work was to verify, during an experimental period of 210 days, the possible effects of the ethanol on the morphology and density of the NADH-diaforase myenteric neurons in the jejunum of alcoholic rats. We used 10 animals (Rattus norvegicus) separated in 2 groups: the controls (n=5), that received a normal proteic diet (22%) and water ad libitum; the alcoholic, that received NUVILAB normal proteic chow (22%) and sugar cane brandy diluted at 30 Gay Lussac (30 v/v). The jejunum segment was collected and submitted to the histochemical technique to stain the nervous cells and, then, to the elaboration of membrane whole mounts. The observation through light microscopy showed that there are no expressive morphologic differences between the...
Tipo: Journal article Palavras-chave: Jejunum; Myenteric neurons; Rats; Alcoholism.
Ano: 2006 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022006000400016
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Morphometry and Quantification of the Myenteric Neurons of the Cecum of Rats (Rattus norvegicus) Submitted to Experimental Chronic Alcoholism International Journal of Morphology
Pereira,Marli Aparecida dos Santos; Miranda Neto,Marcílio Hubner; Arantes,Márcia Alessandra; Molinari,Sonia Lucy; Pereira,Priscila; Zanoni,Jacqueline Nelisis.
Morphometric and quantitative analyses were accomplished to study the effects of the consumption of alcohol above the size and density of the myenteric neurons of cecum of rats. Ten rats with 90 days were divided in the groups: control (C), and alcoholic (A). After 120 days of treatment with ethanol the cecum of both groups were collected, submitted it prepared of membranes that after carried out to Giemsa´s technique, permitted to evaluate the neuronal density, in an area of 13.44mm², and measure the cell body area of 300 neurons by group. The alcoholic rats presented an increase in the number of small neurons and a reduction of the big and medium neurons. The neuronal density verified in alcoholic rats was significantly reduced regarding the controls...
Tipo: Journal article Palavras-chave: Alcoholism; Cecum; Myenteric neurons.
Ano: 2009 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022009000300001
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DENSITY AND MORPHOMETRY OF MYENTERIC NEURONS OF THE ILEUM OF RATS SUBJECTED TO CHRONIC ALCOHOLISM International Journal of Morphology
dos Santos Pereira,Marli Aparecida; Molinari,Sonia Lucy; de Sousa,Fernando Carlos; André,Oriana Elaine; de Miranda Neto,Marcílio Hubner.
This work verified the changes caused by ethanol ingestion on the myenteric neurons of the ileum of rats subjected to chronic alcoholism. Twenty rats (Rattus norvegicus) were divided in control (GC) and alcoholic (GA) groups during 210 days. The animals from group GA received sugar-cane brandy diluted to 30 GL and those from group GC received water. Both groups were fed with rodent chow ad libitum. The ileum was prepared as whole-mounts for neuronal staining according to Barbosa (1978) and Gabella (1979). The neurons were counted and the cell bodies were measured. The statistical analysis revealed a significant decrease in the number of large neurons in GA compared with GC, while there was a significant increase in the number of small neurons in GA. There...
Tipo: Journal article Palavras-chave: Alcoholism; Ileum; Myenteric neurons.
Ano: 2003 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-95022003000300010
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Morphoquantitative evaluation of the duodenal myenteric neuronal population in rats fed with hypoproteic ration Biocell
Marçal Natali,María Raquel; Molinari,Sonia Lucy; Valentini,Luiz Cristiano; Hubner de Miranda Neto,Marcílio.
The purpose of this work was to analyze the morphoquantitative behavior of the neurons of the myenteric plexus, as well as the morphometry of the duodenal wall, in adult rats fed with normoproteic (22%) and hypoproteic (8%) rations, killed at the age of 345 days. For neuronal assessments duodenal wholemounts stained with the Giemsa method were used, and for the evaluation of the duodenal wall routine histological processing and staining with Hematoxilin-Eosin were employed. The means of the number of neurons in 80 microscopic fields (12.72 mm2) and of the size of the neuronal cell bodies did not reveal statistically significant differences between the groups, but there was a greater incidence of large neurons in the protein restriction group (RP). The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Myenteric neurons; Small intestine; Morphometric; Hypoproteic ration.
Ano: 2005 URL: http://www.scielo.org.ar/scielo.php?script=sci_arttext&pid=S0327-95452005000100006
Registros recuperados: 5
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