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Technology and safety assessment for lactic acid bacteria isolated from traditional Bulgarian fermented meat product "lukanka" BJM
Todorov,Svetoslav Dimitrov; Stojanovski,Saso; Iliev,Ilia; Moncheva,Penka; Nero,Luis Augusto; Ivanova,Iskra Vitanova.
Abstract The present work discusses the technological and new selection criteria that should be included for selecting lactic acid bacteria for production of fermented meat. Lactic acid bacteria isolated from Bulgarian traditional fermented "lulanka" salami was studied regarding some positive technological parameters (growth at different temperature, pH, and proteolytic activity). The presence of genes related to the virulence factors, production of biogenic amines, and vancomycin resistance were presented in low frequency in the studied lactic acid bacteria. On the other hand, production of antimicrobial peptides and high spread of bacteriocin genes were broadly presented. Very strong activity against L. monocytogenes was detected in some of the studied...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lukanka; Antibiotic resistance; Virulence factors; Lactic acid bacteria; Bacteriocins.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000300576
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Molecular methods for diversity assessment among xanthomonads of Bulgarian and Macedonian pepper BJM
Vancheva,Taca; Stoyanova,Mariya; Tasheva-Terzieva,Elena; Bogatzevska,Nevena; Moncheva,Penka.
ABSTRACT Bacterial spot is an important disease of pepper in Bulgaria and Macedonia. For characterization of Xanthomonas species associated with bacterial spot, 161 strains were collected from various field pepper-growing regions. Among them, 131 strains were identified as Xanthomonas euvesicatoria and 30 as Xanthomonas vesicatoria using species-specific primers and polymerase chain reaction followed by restriction fragment length polymorphism analysis. To assess the genetic diversity of the strains, two methods (Random Amplified Polymorphic DNA and Repetitive Element Palindromic-Polymerase Chain Reaction) were applied. Discriminatory index was calculated and analysis of molecular variance was carried out.Combined random amplified polymorphic DNA analysis...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial spot; RFLP; RAPD; REP-PCR; Heterogeneity.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000500246
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