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Study on Thermodynamics and Adsorption kinetics of Purified endoglucanase (CMCase) from Penicillium notatum NCIM NO-923 produced under mixed solid-state fermentation of waste cabbage and Bagasse BJM
Das,Arpan; Ghosh,Uma; Mohapatra,Pradeep Kumar Das; Pati,Bikas Ranjan; Mondal,Keshab Chandra.
In the current study, one thermostable endoglucanase was purified from Penicillium notatum NCIM NO-923 through mixed solid state fermentation of waste cabbage and bagasse. The molecular weight of the purified enzyme was 55kDa as determined by SDS polyacrylamide gel electrophoresis (SDS-PAGE). The enzyme had low activation energy (Ea) of 36.39KJ mol-1 for carboxymethyl cellulose hydrolysis and the enthalpy and entropy for irreversible inactivation was 87 kJ mol −1 and 59.3 J mol −1 K−1 respectively. The enzyme was quite thermostable with a Tm value of 62.2˚C. The pKa1 and pKa2 of ionizable groups of the active sites were 2.5 and 5.3 respectively. Apparent Km, Vmax and Kcat of the enzyme were found to be 5.2 mg mL-1, 80 U/gds and 322.4 sec-1 respectively....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endoglucanase; Langmuir adsorption isotherm; Penicillium notatum; Solid state fermentation.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300037
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Rapid screening of tannase producing microbes by using natural tannin BJM
Jana,Arijit; Maity,Chiranjit; Halder,Suman Kumar; Pati,Bikas Ranjan; Mondal,Keshab Chandra; Mohapatra,Pradeep Kumar Das.
Use of natural tannin in the screening of tannase producing microbes is really promising. The present work describes about the possibility and integrity of the newly formulated method over the previously reported methods. Tannin isolated from Terminalia belerica Roxb. (Bahera) was used to differentiate between tanninolytic and nontanninolytic microbes. The method is simple, sensitive and superior for the rapid screening and isolation of tannase-producing microbes.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Natural tannin; Bahera; Tannase; Plate screening; Enteric yeast.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300034
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