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	Provedor de dados BJMBR (2) Autor Monesi,N. (2) Candido-Silva,J.A. (1) Paçó-Larson,M.L. (1) Sousa,J.F. (1) Palavra-chave Bradysia hygida (1) DNA puff (1) Development (1) Ecdysone (1) Eukaryotic Elongation Factor 1A (1) Gene expression (1) Polytene chromosomes (1) Sciaridae (1) Transgenic Drosophila (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2010 (1) 2001 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Bradysia hygida (Diptera, Sciaridae) presents two eukaryotic Elongation Factor 1A gene homologues: partial characterization of the eukaryotic Elongation Factor 1A-F1 gene BJMBR Candido-Silva,J.A.; Monesi,N.. Elongation factor 1A is a highly conserved protein that participates in translation. We report the occurrence of two genes homologous to the eukaryotic Elongation Factor 1A in Bradysia hygida and describe the partial cloning and characterization of the B. hygida eukaryotic Elongation Factor 1A-F1 (BheEF1A-F1) gene. The pattern of BheEF1A-F1 expression in the salivary gland at the end of the fourth larval instar was investigated using real-time PCR. The results showed that BheEF1A-F1 expression levels are relatively constant at the time when rapid changes in protein synthesis occur in this tissue. In situ hybridization experiments coupled to Southern blot analyses showed that the BheEF1A-F1 gene is located at position 3d of the A chromosome and a second... Tipo: Info:eu-repo/semantics/article Palavras-chave: Eukaryotic Elongation Factor 1A; Bradysia hygida; Polytene chromosomes; Sciaridae. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2010000500004 The DNA puff BhB10-1 gene is differentially expressed in various tissues of Bradysia hygida late larvae and constitutively transcribed in transgenic Drosophila BJMBR Monesi,N.; Sousa,J.F.; Paçó-Larson,M.L.. We extended the characterization of the DNA puff BhB10-1 gene of Bradysia hygida by showing that, although its mRNA is detected only at the end of the fourth larval instar, BhB10-1 expression is not restricted to the salivary gland, the tissue in which this gene is amplified. Different amounts of BhB10-1 mRNA were detected in other larval tissues such as gut, Malpighian tubules, fat body, brain and cuticle, suggesting that this gene is expressed differentially in the various tissues analyzed. Analysis of transgenic Drosophila carrying the BhB10-1 transcription unit and flanking sequences revealed that the tested fragment promotes transcription in a constitutive manner. We suggest that either cis-regulatory elements are missing in the transgene or factors... Tipo: Info:eu-repo/semantics/article Palavras-chave: DNA puff; Gene expression; Development; Ecdysone; Transgenic Drosophila. Ano: 2001 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-879X2001000700003 Registros recuperados: 2 Primeira ... 1 ... Última

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