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	Provedor de dados Ciência Rural (1) Autor Brandão,Fernanda de Lima (1) Brito,Harald Fernando Vicente de (1) Fernandes-Filho,Tobias (1) Laskoski,Luciane Maria (1) Locatelli-Dittrich,Rosangela (1) Montaño,Patrícia Yukiko (1) Mais... Palavra-chave Immunophenotyping (1) Mesenchymal stem cells (1) Multipotent stem cell (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2015 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Anticoagulants on yield of bone marrow-derived mononuclear cells harvested from dogs Ciência Rural Brito,Harald Fernando Vicente de; Fernandes-Filho,Tobias; Brandão,Fernanda de Lima; Montaño,Patrícia Yukiko; Laskoski,Luciane Maria; Locatelli-Dittrich,Rosangela. Cell therapy with bone marrow-derived mononuclear cells is an alternative to therapy with mesenchymal stem cell cultures. The aim of the present research was the comparison of the yield of bone marrow-derived mononuclear cells harvested from dogs with two different anticoagulants. Bone marrow was harvested from the iliac crest of five healthy dogs aged between 15 and 30 months, and the effect of two anticoagulant solutions, CPDA-1 (citrate phosphate dextrose adenine-1) and heparin, on the isolation of mononuclear cells was compared. Mononuclear cells were isolated in a density gradient and stained for CD9 and CD44 for characterization by flow cytometry. Means were compared using Student's paired t-test. Samples harvested with CPDA-1 yielded an average of... Tipo: Info:eu-repo/semantics/article Palavras-chave: Mesenchymal stem cells; Multipotent stem cell; Immunophenotyping. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015001102044 Registros recuperados: 1 Primeira ... 1 ... Última

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