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	Provedor de dados ArchiMer (3) Autor Montagu, Monique (3) Amiard, Jean-claude (2) Mondeguer, Florence (2) Poirier, Laurence (2) Quiniou, Francoise (2) Ruiz, Nicolas (2) Maillols, Hélène (1) Mestres, Gilberte (1) Pouchus, Yves Francois (1) Pouchus, Yves François (1) Rouland, Caroline (1) Mais... Palavra-chave Acide okadaïque (1) Alternative method (1) Automation (1) Automatisation (1) Bivalve bioassay (1) Couplage CLHP/ESI/SM2 par piège d’ions. (1) Dinophysistoxin-1 (1) Dinophysistoxine-1 (1) Dinophysistoxines (1) Dinophysistoxins (1) Electrospray ionization ion trap mass spectrometry ESI IT MS (1) Embryotoxicity (1) Extraction sur phase solide (1) Fungal peptide metabolites (1) Intoxication des coquillages (1) Liquid chromatography coupled ion trap. (1) Marine fungal contamination (1) Marine fungi (1) Matched calibration (1) Matrix (1) Mais... Tipo do documento Text (3) Ano 2007 (2) 2003 (1) País France (3) Idioma Inglês (2) Francês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Toxicity assessment of peptaibols and contaminated sediments on Crassostrea gigas embryos ArchiMer Poirier, Laurence; Quiniou, Francoise; Ruiz, Nicolas; Montagu, Monique; Amiard, Jean-claude; Pouchus, Yves François. Peptaibols are known membrane-modifying peptides that were recently detected in marine sediments and mussels collected from a shellfish farming area (Fier d'Ars, Atlantic coast, France). In this investigation, embryotoxicity bioassays with oysters (Crassostrea gigas) were performed to assess acute toxicity of alamethicin and different groups of peptaibols produced by a Trichoderma longibrachiatum strain isolated from marine environment. C gigas embryos appeared very sensitive to all the metabolites examined with higher toxic effects for long-sequence peptides (EC50 ranging from 10 to 64nM). D-shaped larvae with mantle abnormality were particularly noticed when peptaibol concentrations increased. Disturbances of embryogenesis were also observed following... Tipo: Text Palavras-chave: Risk assessment; Embryotoxicity; Bivalve bioassay; Mycotoxins; Marine fungi. Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2680.pdf Determination of peptaibol trace amounts in marine sediments by liquid chromatography/electrospray ionization-ion trap-mass spectrometry ArchiMer Poirier, Laurence; Amiard, Jean-claude; Mondeguer, Florence; Quiniou, Francoise; Ruiz, Nicolas; Pouchus, Yves Francois; Montagu, Monique. Extraction followed by reverse phase liquid chromatography (LC)/electrospray ionization-ion trap-mass spectrometry (ESI-IT-MS) analysis has been successfully developed for the determination of peptaibols, fungal toxic metabolites, in marine sediments. Spiking experiments showed that the mean recovery of target compounds exceeded 85% at a spiking level of 10 ng/g of sediment (wet weight). Detection and quantification limits were 250 and 830 pg/g of sediment, respectively. The method developed constituted the first sensitive assay for quantification of peptaibol trace amounts in a natural environment. A concentration of 5 ng/g in sediment samples collected from Fier d'Ars was found. Tipo: Text Palavras-chave: Matched calibration; Matrix; Electrospray ionization ion trap mass spectrometry ESI IT MS; Marine fungal contamination; Trichoderma sp.; Fungal peptide metabolites. Ano: 2007 URL: http://archimer.ifremer.fr/doc/2007/publication-2990.pdf Validation d’un procédé automatisé d’extraction sur phase solide de glandes digestives de moules pour l’identification et la quantification des dinophysistoxines en LC/ESI/SM2 par piégeage d’ions quadripolaire ArchiMer Mondeguer, Florence; Mestres, Gilberte; Rouland, Caroline; Montagu, Monique; Maillols, Hélène. This report presents a new extraction method of the dinophysistoxins (DTXs), confirmed by quantification using high-performance liquid chromatography coupled to mass spectrometry with an ion trap and electro spray interface (HPLC/ESI/MS2). The method originality consists on the adaptation of DTXs basic extraction procedure (liquid/ liquid) to a solid phase extraction (SPE) via a robotic station: ASPEC XLi The parameters of the automatization procedure were optimized to obtain the best DTXs recovery rate. These improvements were loaded with digestive gland mussel homogenat realized on a silica cartridge SPE, activated in hexane/chloroform (50:50), washed with hexane/chloroform (50:50) and extracted by an elution gradient (chloroform methanol (65:35) and... Tipo: Text Palavras-chave: Toxines; Intoxication des coquillages; Dinophysistoxines; Acide okadaïque; Dinophysistoxine-1; Méthode alternative; Extraction sur phase solide; Automatisation; Couplage CLHP/ESI/SM2 par piège d’ions.; Toxins; Shellfish poisoning; Dinophysistoxins; Okadaic acid; Dinophysistoxin-1; Alternative method; Solid phase extraction; Automation; Liquid chromatography coupled ion trap.. Ano: 2003 URL: http://archimer.ifremer.fr/doc/00342/45270/44724.pdf Registros recuperados: 3 Primeira ... 1 ... Última

Empresa Brasileira de Pesquisa Agropecuária - Embrapa Todos os direitos reservados, conforme Lei n° 9.610 Política de Privacidade Área restrita		Embrapa Parque Estação Biológica - PqEB s/n° Brasília, DF - Brasil - CEP 70770-901 Fone: (61) 3448-4433 - Fax: (61) 3448-4890 / 3448-4891 SAC: https://www.embrapa.br/fale-conosco