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| Barbosa,PSF; Martins,AMC; Toyama,MH; Joazeiro,PP; Beriam,LOS; Fonteles,MC; Monteiro,HSA. |
| Snake venom proteins from the C-type lectin family have very distinct biological activities despite their highly conserved primary structure, which is homologous to the carbohydrate recognition region of true C-type lectins. We purified a lectin-like protein (BmLec) from Bothrops moojeni venom and investigated its effect on platelet aggregation, insulin secretion, antibacterial activity, and isolated kidney cells. The BmLec was purified using two chromatographic steps: affinity chromatography and reverse phase high performance liquid chromatography (HPLC). BmLec showed a dose-dependent platelet aggregation and significantly decreased the bacterial growth rate in approximately 15%. During scanning electron microscopy, the profile of Xanthomonas axonopodis... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Bothrops moojeni; Kidney; Platelet aggregation; Insulin; Antibacterial activity. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000300016 |
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| Vinhote,JFC; Torres,AFC; Dantas,RT; Praciano,TP; Menezes,RRPPB; Sousa,DF; Brito,TS; Lima,FJB; Toyama,MH; Magalhães,PJ; Monteiro,HSA; Martins-Nunes,AMC. |
| In the present study, the effects of Polybia paulista venom (PPV) on renal and vascular tissues were investigated. Isolated kidneys perfused with PPV (1 and 3 μg/mL) had increased perfusion pressure, renal vascular resistance, urinary flow, and glomerular filtration rate; and reduced sodium tubular transport. Histological evaluation demonstrated deposits of proteins in Bowman's space and tubular lumen, and focal areas of necrosis. The venom promoted a cytotoxic effect on Madin-Darby canine kidney (MDCK) cells. A significant increase in lactic dehydrogenase levels was observed in response to venom exposure. In isolated mesenteric vascular beds, pressure and vascular resistance augmented in a dose-dependent manner. PPV increased the contractility of aortic... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Polybia paulista; Venom; Kidney; Aorta; MDCK. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000200011 |
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| Torres,AFC; Dantas,RT; Menezes,RRPPB; Toyama,MH; Filho,ED; Oliveira,MF; Nogueira,NAP; Oliveira,MR; Monteiro,HSA; Martins,AMC. |
| Some snake venom proteins present enzymatic activities, such as L-amino acid oxidase (LAAO). The aim of this paper was to investigate the effect of Bothrops leucurus total venom (BleuTV) and its fraction LAAO (BleuLAAO) on bacteria, yeast, and promastigote forms of Leishmania amazonensis and Leishmania chagasi, and epimastigote forms of Trypanosoma cruzi. BleuTV was isolated with a Protein Pack 5PW® (Waters Corporation, USA), and several fractions were obtained. BleuLAAO was purified to high molecular homogeneity, and its N-terminal amino acid sequence shared a high degree of amino acid conservation with other LAAOs. BleuTV inhibited Staphylococcus aureus growth in a dose-dependent manner, with a minimum inhibitory concentration (MIC) of 25 μg/mL, which... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: L-amino oxidase; Bothrops leucurus; Antimicrobial activity. |
| Ano: 2010 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400012 |
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| Martins,AMC; Barbosa,PSF; Sousa,DF; Alves,CD; Menezes,DB; Lima,C; Lopes-Ferreira,M; Fonteles,MC; Monteiro,HSA. |
| Thalassophryne nattereri (niquim) is a venomous fish responsible for numerous accidents involving fishermen in northern and northeastern Brazil. The aim of the present investigation was to evaluate the action of antivenom on renal effects caused by Thalassophryne nattereri venom. Isolated kidneys of Wistar rats were perfused with a previously dialyzed Krebs-Henseleit solution containing 6 g% bovine serum albumin. The antivenom action was studied through perfusion pressure (PP), renal vascular resistance (RVR), urinary flow (UF) and glomerular filtration rate (GFR). The niquim venom (1 µg/mL), the antivenom alone (1 µg/mL) or the venom incubated with antivenom were added to the system 30 minutes after the beginning of each perfusion. Previous works have... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Thalassophryne nattereri; Antivenom; Kidney. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992009000100011 |
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| Pereira,TP; Bezerra de Menezes,RRPP; Torres,AFC; Brito,TS; Batista-Lima,FJ; Vinhote,JFC; Sousa,DF; Ximenes,RM; Toyama,MH; Diz Filho,EBS; Magalhães,PJC; Monteiro,HSA; Martins,AMC. |
| In this study, we evaluated the actions of Crotalus durissus cumanensis venom (CDCmV), and its crotoxin (Crtx) fraction, on renal and vascular functions in Wistar rats. In isolated perfused kidneys, CDCmV (10 µg/mL) significantly increased the perfusion pressure (PP) from 110.7 ± 2.4 to 125.3 ± 2.8 mmHg after 30 minutes. This effect was accompanied by an increased renal vascular resistance (RVR) from 5.4 ± 0.1 to 6.2 ± 0.2 mmHg/mL.g-1.min-1. We observed decreases in urinary flow (UF) from 0.13 ± 0.01 to 0.05 ± 001 mL.g-1.min-1 and glomerular filtration rate (GFR) from 0.66 ± 0.06 to 0.18 ± 0.02 mL.g-1.min-1. Crtx did not change PP or RVR, but diminished GFR (from 0.65 ± 0.05 to 0.26 ± 003 mL.g-1.min-1) and UF (from 0.11 ± 0.008 to 0.09 ± 0.008... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Kidney; Vascular injuries; Crotalus durissus cumanensis; Crotoxin. |
| Ano: 2011 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992011000300014 |
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