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Determining the Best Sectioning Method and Intestinal Segment for Morphometric Analysis in Broilers Rev. Bras. Ciênc. Avic.
Gava,MS; Moraes,LB; Carvalho,D; Chitolina,GZ; Fallavena,LCB; Moraes,HLS; Herpich,J; Salle,CTP.
Brazilian poultry production is very efficient and demands maximum broiler performance. Therefore, digestive system pathologies have a relevant role. Considering it is difficult to obtain consistent information on intestinal morphometric analysis, this study aimed at establishing essential and clear criteria for the collection of intestinal segments for morphometric analysis. Fifteen 13-d-old broilers were sacrificed and three intestinal segments were collected per bird. Two 3-cm long sections were obtained from each of the intestinal segments. Samples were collected open or closed. The closed samples were transversely, hemicylindrically, or longitudinally sectioned. Samples were processed and stained with hematoxylin and eosin. The number of microscopic...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Image; Intestine; Morphometry; Poultry; Villus.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000200145
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Infectious Bursal Disease: Pathogenicity and Immunogenicity of Vaccines Rev. Bras. Ciênc. Avic.
Camilotti,E; Moraes,LB; Furian,TQ; Borges,KA; Moraes,HLS; Salle,CTP.
ABSTRACT The Infectious Bursal Disease (IBD) is a contagious viral disease that affects young chickens and may cause high morbidity and mortality. As the virus is very resistant to the environment, vaccination is required in case of high infection pressure. Due to variations in the virulence degree of the vaccines available to control IBD, this study aimed at evaluating the pathogenicity and immunogenicity of three types of vaccines. In total, 220 one-day-old specific pathogen free (SPF) chickens were immunized with recombinant, immune-complex and intermediate vaccines, or not vaccinated (55 birds per group) and challenged with IBD G11 strain on day 25. On days 25, 30, and 35, the Bursa of Fabricius (BF) were submitted to gross and histological...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Infectious Bursal Disease; Chickens; Vaccine; Immunity.
Ano: 2016 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2016000200303
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