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Evaluation of Petrifilm™ EC and HS for total coliforms and Escherichia coli enumeration in water BJM
Beloti,Vanerli; Souza,Juliana Aparecida de; Barros,Márcia de Aguiar Ferreira; Nero,Luís Augusto; Mattos,Marcos Rodrigues de; Gusmão,Viviane Vieira; Moraes,Luciane Bilia de.
Petrifilm™ EC and HS were compared to the MPN method to determine their efficacy to enumerate total coliforms and E. coli in 145 samples of water (76 water in natura, 69 drinking water). For water in natura, Petrifilm™ HS and EC showed good correlation with MPN method. In chlorinated water (< 20 col/100 mL and negatives) the agreement was low. For E.coli enumeration, Petrifilm™ EC showed a good correlation with MPN method. The results indicated that Petrifilm™ EC and HS are accurate to enumerate coliforms and E. coli in water when the expected counts are higher than 20/100 mL.
Tipo: Info:eu-repo/semantics/other Palavras-chave: Water; Coliforms; Escherichia coli; Petrifilm™.
Ano: 2003 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822003000400002
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Assessment of the efficiency of SimPlate™ total plate count color indicator (TPC CI) to quantify mesophilic aerobic microorganisms in pasteurized milk BJM
Nero,Luís Augusto; Beloti,Vanerli; Barros,Márcia de Aguiar Ferreira; Santana,Elsa Helena Walter de; Pereira,Mykel Steffani; Gusmão,Viviane Vieira; Moraes,Luciane Bilia de.
The SimPlate™ TPC CI system is a rapid method to count mesophilic aerobic microorganisms (MAM) in foods, based on the use of resazurine to indicate bacterial growth. Its efficiency in pasteurized milk was evaluated using 142 pasteurized milk samples (38 type A, 43 type B and 61 type C) collected in Londrina, PR. The standard plating method, using Plate Count Agar (PCA) was used for comparison. The plates of both systems were incubated at 35ºC and read after 24h and 48h. The occurrence of false-positive and false-negative wells and the predominant microorganisms in them were also evaluated. The results were compared by simple correlation and mean variance analyses. The correlation (r) and mean variance values were 0.6811 and 0.7583 for the results obtained...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Rapid methods; Milk; SimPlate.
Ano: 2002 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000100009
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