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	Provedor de dados BJID (1) BJM (1) Rev. Microbiol. (1) Autor Morais,Marcia Maria Camargo de (3) Lima Filho,José Luiz de (2) Maia,Maria de Mascena Diniz (2) Alves,Lilian Rodrigues (1) Bezerra Neto,João Pacífico (1) Jacomé,Paula Regina Luna de Araújo (1) Knight,Kirsty (1) Ledingham,William M. (1) Lima,Jailton Lobo da Costa (1) Maciel,Maria Amélia Vieira (1) Melo,Eduardo Henrique Magalhães (1) Morais Jr.,Marcos Antonio de (1) Pimentel,Maria do Carmo Barros (1) Mais... Palavra-chave Enzyme kinetics (2) Biofilm (1) Dacron (1) Ester hydrolysis (1) Extracellular lipase (1) Fusarium solani (1) Fusarium solani FS1 (1) Immobilized lipase (1) Multiresistance (1) Polyacrylamide beads (1) Pseudomonas aeruginosa (1) Quorum sensing (QS) (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Info:eu-repo/semantics/other (1) Ano 2018 (1) 2000 (1) 1999 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Biofilm production by clinical isolates of Pseudomonas aeruginosa and structural changes in LasR protein of isolates non biofilm-producing BJID Lima,Jailton Lobo da Costa; Alves,Lilian Rodrigues; Jacomé,Paula Regina Luna de Araújo; Bezerra Neto,João Pacífico; Maciel,Maria Amélia Vieira; Morais,Marcia Maria Camargo de. ABSTRACT Introduction: Biofilm production is an important mechanism for the survival of Pseudomonas aeruginosa and its relationship with antimicrobial resistance represents a challenge for patient therapeutics. P. aeruginosa is an opportunistic pathogen frequently associated to nosocomial infections, especially in imunocompromised hosts. Objectives: Analyze the phenotypic biofilm production in P. aeruginosa isolates, describe clonal profiles, and analyze quorum sensing (QS) genes and the occurrence of mutations in the LasR protein of non-biofilm producing isolates. Methods: Isolates were tested for biofilm production by measuring cells adherence to the microtiter plates. Clonal profile analysis was carried out through ERIC-PCR, QS genes were by specific... Tipo: Info:eu-repo/semantics/article Palavras-chave: Pseudomonas aeruginosa; Biofilm; Multiresistance; Quorum sensing (QS). Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-86702018000200129 Immobilization of lipase from Fusarium solani FS1 BJM Knight,Kirsty; Pimentel,Maria do Carmo Barros; Morais,Marcia Maria Camargo de; Ledingham,William M.; Lima Filho,José Luiz de; Maia,Maria de Mascena Diniz. Lipase from Fusarium solani FS1 was immobilized by covalent attachment to polyacrylamide beads and onto magnetized Dacron, retaining 12% and 97% of activity, respectively. Lipase was also entrapped within polyacrylamide beads, retaining 53% of activity. Investigations of the kinetic characteristics of the immobilized derivatives using triolein as substrate showed that lipase immobilized onto polyacrilamide beads and Dacron did not follow Michaelis-Menten kinetics. Tipo: Info:eu-repo/semantics/other Palavras-chave: Fusarium solani FS1; Immobilized lipase; Enzyme kinetics; Dacron; Polyacrylamide beads. Ano: 2000 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822000000300013 Production of extracellular lipase by the phytopathogenic fungus Fusarium solani FS1 Rev. Microbiol. Maia,Maria de Mascena Diniz; Morais,Marcia Maria Camargo de; Morais Jr.,Marcos Antonio de; Melo,Eduardo Henrique Magalhães; Lima Filho,José Luiz de. A Brazilian strain of Fusarium solani was tested for extracellular lipase production in peptone-olive oil medium. The fungus produced 10,500 U.l-1 of lipase after 72 hours of cultivation at 25oC in shake-flask at 120 rpm in a medium containing 3% (w/v) peptone plus 0.5% (v/v) olive oil. Glucose (1% w/v) was found to inhibit the inductive effect of olive oil. Peptone concentrations below 3% (w/v) resulted in a reduced lipase production while increased olive oil concentration (above 0.5%) did not further stimulate lipase production. The optimum lipase activity was achieved at pH 8.6 and 30oC and a good enzyme stability (80% activity retention) was observed at pH ranging from 7.6 to 8.6, and the activity rapidly dropped at temperatures above 50oC. Lipase... Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzyme kinetics; Ester hydrolysis; Extracellular lipase; Fusarium solani. Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400003 Registros recuperados: 3 Primeira ... 1 ... Última

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