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| Batista,Carolina de Sousa Américo; Azevedo,Sérgio Santos de; Alves,Clebert José; Vasconcellos,Sílvio Arruda; Morais,Zenaide Maria de; Clementino,Inácio José; Lima,Fabiano de Silva; Araújo Neto,José Othon de. |
| O presente trabalho teve como objetivo investigar a prevalência de leptospirose em cães errantes da cidade de Patos, Estado da Paraíba, Brasil. O experimento foi conduzido durante o período de fevereiro a abril de 2003, com a colheita de 130 amostras de soro canino. O diagnóstico da leptospirose foi determinado pela técnica de soroaglutinação microscópica, utilizando-se uma coleção de 22 variantes sorológicas. Para a caracterização dos sorovares mais prováveis, levou-se em conta a titulação e a freqüência. A prevalência encontrada foi de 20%, com maior frequência dos sorovares autumnalis (20%), pomona (17,5%), grippotyphosa (10%) e patoc (10%). |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Prevalência; Leptospirose; Cães errantes; Soroaglutinação microscópica. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1413-95962004000200009 |
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| Soto,Francisco Rafael M.; Azevedo,Sérgio Santos de; Morais,Zenaide Maria de; Pinheiro,Sônia Regina; Delbem,Ádina Cléia B.; Moreno,Andréa Micke; Paixão,Renata; Vuaden,Erlete R.; Vasconcellos,Sílvio Arruda. |
| Leptospirosis is an important zoonosis that causes reproductive disorders in swine. The isolation of leptospires from aborted fetuses, stillbirths and weak piglets was obtained in several occasions, however, the bacteria was never isolated from apparently healthy piglets born from apparently healthy infected dams. Six sows of the Landrace breed with a known date of service and pregnancy confirmed by ultrasonography were infected intravenously with 5ml of Leptospira interrogans serovar Canicola inoculum between 76 and 90 days of gestation, and one week after farrowing, at least one piglet per sow was euthanized and samples of liver, kidneys, lungs, heart, spleen and gastric content were taken and examined by PCR. Reproductive disorders or any clinical sign... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Experimental swine leptospirosis; Leptospira spp.; Serovar Canicola; Vertical transmission. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822006000400034 |
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| Dib,Cristina Corsi; Gonçales,Amane Paldês; Morais,Zenaide Maria de; Souza,Gisele Oliveira de; Miraglia,Fabiana; Abreu,Patricia Antonia Estima; Vasconcellos,Silvio Arruda. |
| We investigated the existence of cross-protection between two anti-leptospirosis monovalent experimental bacterins produced with two strains of Leptospira serogroup Pomona: Fromm strain of serovar Kennewicky, isolated from pigs in the United States, and strain GR6 of serovar Pomona isolated from pigs in Brazil. Both were added of aluminum hydroxide as an adjuvant. Experimental bacterins were tested with the hamster potency test in order to assess protection provided against the disease and against the establishment of kidney infection. Controls were polyvalent commercial vaccine produced with Leptospira strains isolated outside Brazil, which included a representative of Pomona serovar, or Sorensen solution added of aluminum hydroxide adjuvant. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pomona; Hamsters; Bacterin; Cross-protection. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000300042 |
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| Tabata,Rosana; Scanavini Neto,Hugo; Zuanaze,Marcelo Alexandre Fagnani; Oliveira,Eugenia Márcia de Deus; Dias,Ricardo Augusto; Morais,Zenaide Maria de; Ito,Fumio Honma; Vasconcellos,Silvio Arruda. |
| Three leptospiral bacterins, produced with different serovars of Serogroup Sejroe, namely the hardjo (bacterin A), wolffi (bacterin B) and guaricura (bacterin C), were evaluated in male hamsters (Mesocricetus auratus) by comparing the agglutinating and neutralizing antibodies titers using microscopic agglutination (MAT) and in vitro growth inhibition (GIT) tests. The immunization schedule was based on two 1.0 mL doses of non-diluted formalininactivated whole culture bacterin given through subcutaneous route with 10-day interval. The challenge was performed ten days after the second vaccine dose, when the animals were inoculated with 0.2 mL of non-inactivated cultures of each serovar through intraperitoneal route. On the 21st post-challenge day (PCD), all... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospira; Bacterins; Hamsters; Neutralizing antibodies; Serogroup Sejroe. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822002000300016 |
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| Sonada,Rafael Bazaglia; Azevedo,Sérgio Santos de; Soto,Francisco Rafael Martins; Costa,Diego Figueiredo da; Morais,Zenaide Maria de; Souza,Gisele Oliveira de; Gonçales,Amane Paldês; Miraglia,Fabiana; Vasconcellos,Sílvio Arruda. |
| Abstract In swine and bovines, leptospirosis prevention and control is carried out via vaccination of susceptible animals using bacterins. However, the efficiency of leptospirosis vaccines has been questioned. This work aimed to investigate the potency of five leptospirosis vaccines sold commercially in Brazil, challenging the animals with one autochthonous strain of Leptospira, Canicola serovar, denoted LO4, isolated from swine. The standard protocol was followed, and renal carriers of Leptospira were identified among the surviving animals by culture and PCR. Of the five vaccines tested, only two proved effective. None of the surviving animals was positive by culture; however, one animal was positive by PCR. Three of the five vaccines sold commercially in... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Leptospirosis; Vaccination; Production animals; Control. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822018000200347 |
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