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| Pino-Lagos,Karina; Michea,Paula; Sauma,Daniela; Alba,Andrea; Morales,Jorge; Bono,María Rosa; Fierro,Alberto; Rosemblatt,Mario. |
| One of the mechanisms for generation of tolerance involves immature dendritic cells (DCs) and a subpopulation of regulatory CD4+ CD25+ T lymphocytes (T REG). The purpose of this work was to analyze how Cyclosporine A (CsA), a widely used immunosuppressive drug, may affect T REG proliferation. Purified and activated murine DCs obtained from bone marrow precursors differentiated with rGMCSF were co-cultured with purified CFSE-labeled T REG from OTII mice, and their phenotype and proliferation analyzed by flow cytometry. Our data indicate that DCs differentiated in the presence of CsA show an altered phenotype, with a lower expression of MHC-II and a lower activating capacity. Additionally, these CsA-treated DCs show decreased production of IL-2 and IL-12 and... |
| Tipo: Journal article |
Palavras-chave: CD4+CD25+ regulatory T cell; Cyclosporine A; Dendritic cells; Transplant tolerance. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0716-97602010000300010 |
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| Hernández,Adrián; Bórquez,Aliro; Alcaíno,Leonardo; Morales,Jorge; Dantagnan,Patricio; Saez,Patricio. |
| The effect of autoclaving on the nutrients' apparent digestibility coefficient (ADC), digestible protein and energy of pea seed meal (P. sativum) fed to Rainbow trout (O. mykiss) was examined. Two samples of the pea meal were autoclaved at 121°C and 1.1 atm for 5 min (5'APM) or 15 min (15'APM), respectively. A third sample, used as control, was not treated (RPM). One reference diet (Basal diet) and 3 experimental diets were elaborated and labelled based on autoclaving time applied to the ingredient (RPM, 5'APM and 15'APM). The four diets were assigned using a completely randomised design, with each treatment having three replicates. 12 tanks were stocked each with 15 trouts with an average weight of 235 ± 10.4 g. Faeces were collected over a 7-day period... |
| Tipo: Journal article |
Palavras-chave: Autoclaving; Apparent digestibility coefficient; Pea seed meal; Rainbow trout. |
| Ano: 2010 |
URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0718-16202010000300003 |
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