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| Registros recuperados: 11 | |
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| Silva,Anna Carolina da; Queiroz,Alana Emilia Soares de França; Porto,Tatiana Souza; Spier,Michele Rigon; Soccol,Carlos Ricardo; Porto,Ana Lúcia Figueiredo; Souza-Motta,Cristina Maria; Moreira,Keila Aparecida. |
| Enzymes obtained by fermentation processes offer a number of advantages and have been widely researched and used throughout the world. This study aimed to partially characterise an inulinase produced from palm and cassava peel. The enzyme was produced via the solid-state fermentation of Aspergillus japonicus URM5633. The optimal temperatures were 50ºC and 55ºC, and the optimal pH values were 5.2 and 3.4 for inulinase fermentatively produced from palm and cassava peel, respectively. The thermostability measurements for inulinase produced in palm showed that the relative activity remained below 100% until 30 minutes of stability for all temperatures, but reached 106.8% at a temperature of 50ºC after 60 minutes. Inulinase from the crude extract of cassava... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Inulinase; Characterisation; Solid-state fermentation; Aspergillus japonicus. |
| Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500005 |
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| Duarte,Ana Rodrigues; Duarte,Débora Maria Rodrigues; Moreira,Keila Aparecida; Cavalcanti,Maria Taciana Holanda; Lima-Filho,José Luiz de; Porto,Ana Lúcia Figueiredo. |
| The partial characterization and purification of milk clotting enzyme obtained from the (root latex) of Jacaratia corumbensis O. kuntze was studied, by fractional precipitation with ammonium sulphate and ion exchange chromatography. The ammonium sulphate precipitate showed five fractions (AS1- 0-20%; AS2 - 20-40%; AS3 - 40-60%; AS4 - 60-80%; AS5 - 80-100%) and among the fractions obtained, the 40-60% fraction (AS3) showed the highest milk clotting activity with a purification factor of 1.2 fold in relation to the crude extract. This fraction when applied on Mono Q column yielded two protein peaks (p1 and p2), but p1 pool showed the best milk-clotting activity. The optimal pH for the crude and partially purified extract was 6.5 and 7.0, respectively. The... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Jacaratia corumbensis O. kuntze; Milk clotting enzyme; Characterization; Purification; Vegetable enzyme. |
| Ano: 2009 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132009000100001 |
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| Silva,Anna Carolina da; Queiroz,Alana Emilia Soares de França; Oliveira,João Tiago Correia; Medeiros,Erika Valente; Souza-Motta,Cristina Maria de; Moreira,Keila Aparecida. |
| Abstract Protein hydrolysates originating from egg white have already been reported to be bioactive and, among their biological activities, possess the antioxidant property that protects the body from early ageing and diseases linked to oxidation. Therefore, the objective of this work was to evaluate the antioxidant activity of hydrolysates obtained by the hydrolysis of egg white from hen poultry. The protease produced by Aspergillus avenaceus URM 6706 was purified and subsequently applied to hydrolysate the egg white, and the degree of hydrolysis was verified during the protease exposure time (4-24 h). The hydrolysis was intensified over time of exposure to the protease. It was possible to detect the antioxidant activities of eliminating the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aspergillus; Purification; ABTS; +; DPPH; Chelators. |
| Ano: 2019 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132019000100508 |
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| Moreira,Keila Aparecida; Cavalcanti,Maria Taciana Holanda; Duarte,Helena Simões; Tambourgi,Elias Basile; Melo,Eduardo Henrique Magalhães de; Silva,Valdinete Lins; Porto,Ana Lúcia Figueiredo; Lima Filho,José Luiz de. |
| The partial characterization of extracellular proteases from Streptomyces clavuligerus NRRL 3585 and 644 mutant was investigated. The enzyme production was carried out in batch fermentation using soy bean filtrate as nitrogen source. Maximum activity was obtained after 96h of fermentation with an initial pH of 7.0. The enzyme was partially purified by ammonium sulphate precipitation. Enzymes from the two strains retained 37% of their initial activities at pH 8.0 after 2 h incubation at 25ºC. Enzyme half-life at pH 8.0 and 60ºC was 40.30 and 53.32 min, respectively for both strains (partially purified extract). The optimum pH was obtained at pH 7.0-8.0 and 8.4 for enzymes produced for 3585 and 644 strains (crude extract), respectively, and 8.4 and 8.0 for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Streptomyces clavuligerus; Extracellular proteases; Thermal stability; PH. |
| Ano: 2001 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822001000300010 |
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| Soares,Elisângela França; Silva,Anna Carolina da; Queiroz,Alana Emilia Soares de França; Gomes,José Erick Galindo; Herculano,Polyanna Nunes; Moreira,Keila Aparecida. |
| Breadfruit is an exotic tree in Brazil, very well acclimatized. Despite of being a laticifer plant, the knowledge about its latex is scarce. However, it is known that proteolytic enzymes represents over 50% of the latex composition in laticifers plants. The aim of this study was to evaluate the potential of breadfruit (Artocarpus altilis var. Apyrena) latex as a source of milk clotting proteases and partially characterize it. The proteolytic activity of the fraction of crude extract was assessed using azocasein and quantitation of total protein, the bicinchoninic acid (BCA). Milk-clotting activity was analyzed using skim milk at 12%. The enzyme activity was analyzed under different temperature conditions (35 to 80°C) and pH (5.8 to 10.7) presenting optimum... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Enzimas coagulantes do leite; Atividade proteolítica; Coagulante vegetal; Fruta-pão. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000100149 |
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| Cruz,Roberta; Ramos,Sérgio Murilo Sousa; Fonseca,Julyanna Cordoville; Motta,Cristina Maria de Souza; Moreira,Keila Aparecida. |
| ABSTRACT The Caatinga biome features an exclusive endemic biodiversity, and is characterized by the presence of xerophytic, deciduous vegetation, high temperatures, and low rainfall. This important park has undergone anthropization, especially through extraction of firewood and timber and growing plants for raising goats. The objectives of this study were to compare the communities of filamentous fungi present in the preserved area and in the anthropized soil of the Catimbau National Park in Buíque, PE, Brazil, and to evaluate the impacts of anthropization on such communities. A total of 12 collections of soil samples were made, six in the preserved area and six in the anthropic area, and the physicochemical properties of the soil samples were analyzed.... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Caatinga; Diversity; Aspergillus; Gongronella; Penicillium. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-06832017000100419 |
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| Registros recuperados: 11 | |
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