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Production and characterization of an enzyme complex from a new strain of Clostridium thermocellum with emphasis on its xylanase activity BJM
Vieira,Werner Bessa; Moreira,Leonora Rios de Souza; Monteiro Neto,Amadeu; Ferreira Filho,Edivaldo Ximenes.
A new bacterial strain (ISO II) was isolated from manure cow and identified as phylogenetically close to the thermophilic cellulolytic bacterium Clostridium thermocellum. The new strain produced extracellular xylanase, pectinase, mannanase and cellulase activities when grown in liquid culture medium containing banana stem as carbon source. The enzyme production profile after growth on banana stem showed that xylanase and cellulase activities were detected in different incubation periods. An enzyme complex containing xylanase, cellulase and mannanase activities was isolated from culture supernatant samples of strainISO II. The complex was partially purified by ultrafiltration and gel filtration chromatography on Sephacryl S-300. Zymogram analysis after...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clostridium thermocellum; Banana stem; Xylanase.
Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000200009
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The hydrolysis of agro-industrial residues by holocellulose-degrading enzymes BJM
Moreira,Leonora Rios de Souza; Ferreira,Gaspar Virgilio; Santos,Sheila Sousa Thurler; Ribeiro,Ana Paula Souza; Siqueira,Félix Gonçalves; Ferreira Filho,Edivaldo Ximenes.
Holocellulose structures from agro-industrial residues rely on main and side chain attacking enzymes with different specificities for complete hydrolysis. Combinations of crude enzymatic extracts from different fungal species, including Aspergillus terreus, Aspergillus oryzae, Aspergillus niger and Trichoderma longibrachiatum, were applied to sugar cane bagasse, banana stem and dirty cotton residue to investigate the hydrolysis of holocellulose structures. A. terreus and A. oryzae were the best producers of FPase and xylanase activities. A combination of A. terreus and A. oryzae extracts in a 50% proportion provided optimal hydrolysis of dirty cotton residue and banana stem. For the hydrolysis of sugar cane bagasse, the best results were obtained with...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agricultural waste; Enzymatic mixtures; Hydrolysis.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000200010
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