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	Provedor de dados BABT (2) BJM (1) Autor Moreira,Mônica (3) Vicente,Vânia Aparecida (3) Glienke,Chirlei (2) Adamoski,Douglas (1) Barbieri,Dicler de Sant'Anna (1) Carvalho,Yanê (1) Gomes,Renata Rodrigues (1) Higuti,llma Hiroko (1) Klisiowicz,Débora do Rocio (1) Najafzadeh,Mohammad Javad (1) Nascimento,Mariana Machado Fidelis do (1) Neiva,Ivana Froede (1) Noschang,Juliana (1) Sun,Jiufeng (1) Mais... Palavra-chave Streptococcus mutans (2) CTAB (1) DNA isolation (1) Genetic variability (1) Padlock probe (1) RAPD (1) RCA (1) Streptococcus (1) Ultrasound (1) Mais... Tipo do documento Info:eu-repo/semantics/article (3) Ano 2015 (1) 2010 (1) 2007 (1) País Brazil (3) Idioma Inglês (3)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 3 Primeira ... 1 ... Última Methodological variations in the isolation of genomic DNA from Streptococcus bacteria BABT Moreira,Mônica; Noschang,Juliana; Neiva,Ivana Froede; Carvalho,Yanê; Higuti,llma Hiroko; Vicente,Vânia Aparecida. In this work, genomic DNA of Streptococcus pyogenes, S. mutans and S. sobrinus was isolated using two methods: either using the detergent cetyltrimethylammonium bromide (CTAB) at 65ºC; or by applying ultrasound to a mixture of silica and celite in CTAB. The composite method that used ultrasound was the more efficient, allowing the straightforward extraction of genomic DNA from Gram-positive bacteria with good quality and reproducibility. Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptococcus; DNA isolation; CTAB; Ultrasound. Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000400013 Detection of Streptococcus mutans using padlock probe based on Rolling Circle Amplification (RCA) BABT Moreira,Mônica; Adamoski,Douglas; Sun,Jiufeng; Najafzadeh,Mohammad Javad; Nascimento,Mariana Machado Fidelis do; Gomes,Renata Rodrigues; Barbieri,Dicler de Sant'Anna; Glienke,Chirlei; Klisiowicz,Débora do Rocio; Vicente,Vânia Aparecida. The aim of this study was to develop and evaluate a padlock probe based on the Rolling Circle Amplification (RCA), which targeted to 16S-23S rDNA region of S. mutans. The specificity of developed padlock probe was tested for DNA within a panel strains, including S. mutans isolated from the saliva and reference strains of the genus Streptococcus, as well as total DNA samples of biofilm and saliva. The results were positive either for DNA samples of S. mutans or DNA samples recovered from the biofilm and saliva revealing the specificity of designed padlock probe. The padlock probe based on the RCA was proved to be an effective, reproducible method for S. mutansdetection and demonstrated the possibility of a rapid detection and accurate identification of S.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Padlock probe; RCA; Streptococcus mutans. Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132015000100054 Genetic variability of Streptococcus mutans isolated from low-income families, as shown by RAPD markers BJM Moreira,Mônica; Vicente,Vânia Aparecida; Glienke,Chirlei. The detection of Streptococcus mutans isolates with high genetic variability in different individuals indicates the occurrence of transmissibility. In this context, nine low-income families (40 individuals in total) with similar social conditions were assessed to identify the bioserotypes of S. mutans using both biochemical and RAPD markers and to establish the degree of similarity among the intra-familial isolates. The polymorphism analysis used the coefficient of Jaccard in both a Principal Coordinates Analysis (PCO) and in a UPGMA clustering method. A total of 157 isolates were obtained from salivary samples, using the morphology of colonies recovered using MSB agar as indicators. From those, 64 were characterized biochemically as S. mutans and 10 as S.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptococcus mutans; Genetic variability; RAPD. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400026 Registros recuperados: 3 Primeira ... 1 ... Última

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