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Registros recuperados: 10
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Physical mapping of the 18S and 5S ribosomal genes in nine Characidae species (Teleostei, Characiformes) Genet. Mol. Biol.
Peres,Wellington Adriano Moreira; Bertollo,Luiz Antonio Carlos; Moreira Filho,Orlando.
Characidae is one of the largest fish families of the Neotropical region, and presenting a pronounced morphological variability, certainly does not constitute a monophyletic group. The cytogenetical data also show a large chromosomal variation and can provide important information for a better understanding of the relationships between the species of this group. 18S and 5S rDNA probes were used in the present study for the chromosomal mapping in different Characidae species from the São Francisco River (Astyanax lacustris, Astyanax scabripinnis, Hasemania nana, Piabina argentea, Orthospinus franciscensis, Serrapinnus heterodon, Serrapinnus piaba and Myleus micans) and Alto Paraná (Astyanax altiparanae) basins. Species with a single pair of chromosomes...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytogenetics; Neotropical fish; Fluorescent in situ hybridization (FISH); RDNA probe.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200009
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Sex chromosome system ZZ/ZW in Apareiodon hasemani Eigenmann, 1916 (Characiformes, Parodontidae) and a derived chromosomal region Genet. Mol. Biol.
Bellafronte,Elisangela; Schemberger,Michelle Orane; Artoni,Roberto Ferreira; Moreira Filho,Orlando; Vicari,Marcelo Ricardo.
Parodontidae fish show few morphological characteristics for the identification of their representatives and chromosomal analyses have provided reliable features for determining the interrelationships in this family. In this study, the chromosomes of Apareiodon hasemani from the São Francisco River basin, Brazil, were analyzed and showed a karyotype with 2n = 54 meta/submetacentric chromosomes, and a ZZ/ZW sex chromosome system. The study revealed active NORs located on pair 11 and additional 18S rDNA sites on pairs 7 and 22. The 5S rDNA locus was found in pair 14. It showed a pericentric inversion regarding the ancestral condition. The satellite DNA pPh2004 was absent in the chromosomes of A. hasemani, a shared condition with most members of Apareiodon....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytogenetics; FISH; 18S rDNA; 5S rDNA; Neotropical fishes.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572012000500010
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Localization of ribosomal genes in three Pimelodus species (Siluriformes, Pimelodidae) of the São Francisco River: 5S genes as species markers and conservation of the 18S rDNA sites Genet. Mol. Biol.
Garcia,Caroline; Moreira Filho,Orlando.
Pimelodidae is one of the most representative of Neotropical catfish families. However, these fish are still poorly studied in terms of cytogenetics, especially regarding the application of more accurate techniques such as the chromosomal localization of ribosomal genes. In the present work, fluorescent in situ hybridization with 5S and 18S rDNA probes was employed for rDNA site mapping in Pimelodus sp., P. fur and P. maculatus from the São Francisco River in the Três Marias municipality - MG. The results from the application of the 18S probe confirmed the previous data obtained by silver nitrate staining, identifying a simple nucleolar organizing region system for these species. However, the labeling results from the 5S rDNA probe demonstrated a...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Pimelodus; Paracentric inversion; 5S rDNA; 18S rDNA; Species marker.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1415-47572008000200018
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Chromosome polymorphism in Ancistrus cuiabae Knaack, 1999 (Siluriformes: Loricariidae: Ancistrini) Neotropical Ichthyology
Mariotto,Sandra; Centofante,Liano; Miyazawa,Carlos S.; Bertollo,Luiz Antonio Carlos; Moreira Filho,Orlando.
Cytogenetic and FISH analyses were performed in 30 Ancistrus cuiabae specimens from a bay near the town of Poconé, in the Pantanal of Mato Grosso, Brazil. The observed diploid number was 2n = 34 chromosomes for both sexes and three distinct katyotypic formulae were found, namely cytotype A (20m, 8sm, 6st, Fundamental Number/FN = 68; 6 males and 11 females), cytotype B (19m, 8sm, 6st, 1a, FN = 67; 8 males and 4 females) and cytotype C (18m, 8sm, 6st, 2a, FN = 66; a single male). NORs's analyses showed that these regions were located in distinct sites on the NOR-bearing chromosome pair, according to cytotypes. Thus, in cytotype A, NORs were located in the terminal region of the short arm of the second metacentric chromosome pair; in cytotype B, they were...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytogenetics; NOR; Pericentric inversion; Chromosomal evolution.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252009000400006
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Chromosome analysis in Saccodon wagneri (Characiformes) and insights into the karyotype evolution of Parodontidae Neotropical Ichthyology
Nirchio,Mauro; Masache,Maria Cecilia; Paim,Fabilene Gomes; Cioffi,Marcelo de Bello; Moreira Filho,Orlando; Barriga,Ramiro; Oliveira,Claudio; Rossi,Anna Rita.
ABSTRACT Parodontidae is a relatively small group of Neotropical characiform fishes consisting of three genera (Apareiodon, Parodon, and Saccodon) with 32 valid species. A vast cytogenetic literature is available on Apareiodon and Parodon, but to date, there is no cytogenetic data about Saccodon, a genus that contains only three species with a trans-Andean distribution. In the present study the karyotype of S. wagneri was described, based on both conventional (Giemsa staining, Ag-NOR, C-bands) and molecular (repetitive DNA mapping by fluorescent in situ hybridization) methods. A diploid chromosome number of 2n = 54 was observed in both sexes, and the presence of heteromorphic sex chromosomes of the ZZ/ZW type was detected. The W chromosome has a terminal...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Ag-NOR; Cytogenetics; FISH; Heterochromatin; ZW sex chromosomes.
Ano: 2021 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252021000100203
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A poorly known case of watershed transposition between the São Francisco and upper Paraná river basins Neotropical Ichthyology
Moreira Filho,Orlando; Buckup,Paulo Andreas.
During construction of the Furnas hydroelectric power dam in the upper rio Paraná basin in the early 1960s, the rio Piumhi drainage outflow was diverted into the headwaters of the São Francisco river basin. The rio Piumhi was a right bank tributary of the rio Grande, which unites with the rio Paranaíba to form the rio Paraná. The transposition allowed the entire fish fauna of the rio Piumhi and associated swamps, lakes, and tributaries to intermingle with the fish fauna of the São Francisco basin via the ribeirão Sujo, a right bank tributary of the São Francisco. The transposition of the watershed may have had a major impact on the current composition of the fish fauna of the upper rio São Francisco watershed.
Tipo: Info:eu-repo/semantics/article Palavras-chave: River transposition.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252005000300015
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Chromosomal distribution of the retroelements Rex 1, Rex 3 and Rex 6 in species of the genus Harttia and Hypostomus (Siluriformes: Loricariidae) Neotropical Ichthyology
Traldi,Josiane B.; Lui,Roberto L.; Martinez,Juliana de F.; Vicari,Marcelo R.; Nogaroto,Viviane; Moreira Filho,Orlando; Blanco,Daniel R..
ABSTRACT The transposable elements (TE) have been widely applied as physical chromosome markers. However, in Loricariidae there are few physical mapping analyses of these elements. Considering the importance of transposable elements for chromosomal evolution and genome organization, this study conducted the physical chromosome mapping of retroelements (RTEs) Rex1, Rex3 and Rex6 in seven species of the genus Harttia and four species of the genus Hypostomus, aiming to better understand the organization and dynamics of genomes of Loricariidae species. The results showed an intense accumulation of RTEs Rex1, Rex3 and Rex6 and dispersed distribution in heterochromatic and euchromatic regions in the genomes of the species studied here. The presence of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Armored catfish; Fluorescence in situ hybridization; Hypostominae; Loricariinae; Transposable elements.
Ano: 2019 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252019000200214
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Cytogenetical analyses in three fish species of the genus Pimelodus(Siluriformes: Pimelodidae) from rio São Francisco: considerations about the karyotypical evolution in the genus Neotropical Ichthyology
Garcia,Caroline; Moreira Filho,Orlando.
Karyotypes and other chromosomal markers were investigated in three species of the catfish genus Pimelodus, namely P. fur, P. maculatus and Pimelodus sp., from municipality of Três Marias, Minas Gerais, Brazil, using differential staining techniques (C-banding, Silver nitrate and CMA3 staining). The diploid chromosome number was 2n = 56 in P. maculatus and Pimelodus sp., while in P. fur 2n = 54. The karyotype of P. fur consisted in 32M + 8SM + 6ST + 8A with fundamental number (NF) of 100, that of P. maculatus 32M + 12SM + 12A with NF = 112, and that of Pimelodus sp. had 32M + 12Sm + 6ST + 6A with NF = 106.The nucleolar organizer regions (NORs) in all three species were invariably detected in telomeres of longer arm of the 20th chromosome pair. These sites...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish cytogenetics; Diploid number reduction; Chromosomal rearrangements; Chromosome banding; Catfishes.
Ano: 2005 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252005000200006
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Karyotypic diversity between allopatric populations of the group Hoplias malabaricus (Characiformes: Erythrinidae): evolutionary and biogeographic considerations Neotropical Ichthyology
Blanco,Daniel Rodrigues; Lui,Roberto Laridondo; Bertollo,Luiz Antonio Carlos; Margarido,Vladimir Pavan; Moreira Filho,Orlando.
Three populations of the group Hoplias malabaricus from the hydrographic basins of the São Francisco, Araguaia/Tocantins and Xingu Rivers in Brazil were analyzed using classic cytogenetic methods (Giemsa staining, C-banding and Ag-NORs) and molecular methods (fluorescent in situ hybridization with 18S rDNA, 5S rDNA and 5SHindIII satellite DNA probes). The chromosome markers allowed the characterization of these populations as belonging to karyomorph A and the detection of inter-population divergences. These differences likely stem from different evolutionary histories resulting from geographic isolation between populations associated to the dispersive mode of these organisms, reinforcing genetic diversity in the group Hoplias malabaricus.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Cytogenetics; Trahira; Karyomorph A; FISH; Geographic isolation.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252010000200015
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The role of chromosomal fusion in the karyotypic evolution of the genus Ageneiosus (Siluriformes: Auchenipteridae) Neotropical Ichthyology
Lui,Roberto Laridondo; Blanco,Daniel Rodrigues; Martinez,Juliana de Fátima; Margarido,Vladimir Pavan; Venere,Paulo Cesar; Moreira Filho,Orlando.
Ageneiosus is the most widely distributed genus of the family Auchenipteridae among South American river basins. Although chromosome studies in the family are scarce, this genus has the largest number of analyzed species, with 2n = 54 to 56 chromosomes, differing from the rest of the family (2n = 58). This study aimed to analyze Ageneiosus inermis from the Araguaia River basin. The diploid number found was of 56 chromosomes. Heterochromatin was allocated in terminal region of most chromosomes, plus a pericentromeric heterochromatic block in pair 1, a pair distinguished by size in relation to other chromosomes pairs. AgNORs were detected in only one submetacentric chromosome pair, which was confirmed by FISH. 5S rDNA was present in only one metacentric...
Tipo: Info:eu-repo/semantics/article Palavras-chave: C banding; Cytogenetic Markers; [GATA]n sequence; RDNA-FISH; [TTAGGG]n sequence.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1679-62252013000200327
Registros recuperados: 10
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