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Characterization of typical and atypical enteropathogenic Escherichia coli (EPEC) strains of the classical O55 serogroup by RAPD analysis Rev. Microbiol.
Girão,Dennys M.; Bando,Sílvia Y.; Girão,Valéria Brígido de C.; Moreira-Filho,Carlos A.; Fracalanzza,Sérgio Eduardo L.; Trabulsi,Luiz R.; Monteiro-Neto,Valério.
The genetic diversity of 41 typical and atypical enteropathogenic Escherichia coli (EPEC) strains of the serogroup O55 was analyzed by using the random amplified polymorphic DNA (RAPD) method. All typical EPEC O55 strains were grouped in two clusters (A and C) and belonged to the serotype O55:H6, while cluster B included all atypical strains, which were of the serotype O55:H7. The three groups also included non-motile strains. RAPD may be a useful method for epidemiological studies on E. coli O55 infection.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enteropathogenic Escherichia coli; EPEC; Genetic diversity; RAPD analysis.
Ano: 1999 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0001-37141999000400013
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Molecular characterization of nitrogen-fixing bacteria isolated from brazilian agricultural plants at São Paulo state BJM
Reinhardt,Érica. L.; Ramos,Patrícia L.; Manfio,Gilson P.; Barbosa,Heloiza R.; Pavan,Crodowaldo; Moreira-Filho,Carlos A..
Fourteen strains of nitrogen-fixing bacteria were isolated from different agricultural plant species, including cassava, maize and sugarcane, using nitrogen-deprived selective isolation conditions. Ability to fix nitrogen was verified by the acetylene reduction assay. All potentially nitrogen-fixing strains tested showed positive hybridization signals with a nifH probe derived from Azospirillum brasilense. The strains were characterized by RAPD, ARDRA and 16S rDNA sequence analysis. RAPD analyses revealed 8 unique genotypes, the remaining 6 strains clustered into 3 RAPD groups, suggesting a clonal origin. ARDRA and 16S rDNA sequence analyses allowed the assignment of 13 strains to known groups of nitrogen-fixing bacteria, including organisms from the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Endophytic bacteria; Diazotrophs; Selective isolation; Molecular systematics.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822008000300002
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