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Hardison, D. Ransom; Holland, William C.; Mccall, Jennifer R.; Bourdelais, Andrea J.; Baden, Daniel G.; Darius, H. Taiana; Chinain, Mireille; Tester, Patricia A.; Shea, Damian; Quintana, Harold A. Flores; Morris, James A., Jr.; Litaker, R. Wayne. |
Ciguatera fish poisoning is an illness suffered by > 50,000 people yearly after consumption of fish containing ciguatoxins (CTXs). One of the current methodologies to detect ciguatoxins in fish is a radiolabeled receptor binding assay (RBA((R))). However, the license requirements and regulations pertaining to radioisotope utilization can limit the applicability of the RBA((R)) in certain labs. A fluorescence based receptor binding assay (RBA((F))) was developed to provide an alternative method of screening fish samples for CTXs in facilities not certified to use radioisotopes. The new assay is based on competition binding between CTXs and fluorescently labeled brevetoxin-2 (BODIPY (R)-PbTx-2) for voltage-gated sodium channel receptors at site 5 instead... |
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Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00664/77575/79555.pdf |
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Hardison, D. Ransom; Holland, William C.; Darius, H. Taiana; Chinain, Mireille; Tester, Patricia A.; Shea, Damian; Bogdanoff, Alex K.; Morris, James A., Jr.; Quintana, Harold A. Flores; Loeffler, Christopher R.; Buddo, Dayne; Litaker, R. Wayne. |
Lionfish, native to reef ecosystems of the tropical and sub-tropical Indo-Pacific, were introduced to Florida waters in the 1980s, and have spread rapidly throughout the northwestern Atlantic, Caribbean Sea and the Gulf of Mexico. These invasive, carnivorous fish significantly reduce other fish and benthic invertebrate biomass, fish recruitment, and species richness in reef ecosystems. Fisheries resource managers have proposed the establishment of a commercial fishery to reduce lionfish populations and mitigate adverse effects on reef communities. The potential for a commercial fishery for lionfish is the primary reason to identify locations where lionfish accumulate sufficient amounts of ciguatoxin (CTX) to cause ciguatera fish poisoning (CFP), the... |
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Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00660/77221/78672.pdf |
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