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Sarcoplasmatic and myofibrillar protein changes caused by acute heat stress in broiler chicken Scientia Agricola
Santos,Carolina de Castro; Delgado,Eduardo Francisquine; Menten,José Fernando Machado; Pedreira,Aparecida Carla de Moura; Castillo,Carmen Josefina Contreras; Mourão,Gérson Barreto; Brossi,Camila; Silva,Iran José Oliveira da.
Acute heat stress (AHS) modifies the structure of myofibrils affecting functional properties of meat, mainly the water holding capacity. This experiment aimed to identify changes in proteolysis and migration between the myofibrillar and sarcoplasmatic fractions due to pre-slaughter AHS. Myofibrillar fragmentation index (MFI), SDS-PAGE, western blot of vinculin (WB) and shear force (SF) were determined. Six hundred broilers (Gallus gallus) were slaughtered in three different days (ST). In each ST, groups of ten animals were placed in transport crates and submitted to AHS (35ºC, 75 - 85% RH) for 2 hours. Simultaneously, the non-stressed broilers (NS) were kept in thermoneutral environment (22ºC, 83 ± 6.6% RH) within the crates in the same density. After...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Broiler; Heat stress; Lightness; Fragmentation index; Shear force.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162008000500002
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