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	Provedor de dados BABT (1) BJM (1) Autor Mrudula,Soma (2) Murugammal,Rangasamy (1) Shyam,Nidhi (1) Palavra-chave Aspergillus niger (1) Cell leakage (1) Cellulase (1) Coir waste (1) Detergent (1) Immobilized cells (1) Optimization (1) Oxidant stability (1) Solid-state fermentation (1) Submerged fermentation (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2012 (1) 2011 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Immobilization of Bacillus megaterium MTCC 2444 by Ca-alginate entrapment method for enhanced alkaline protease production BABT Mrudula,Soma; Shyam,Nidhi. Optimization of culture conditions and immobilization parameters for alkaline protease production was carried out by employing Bacillus megaterium MTCC2444. The partially purified enzyme was tested for its stability in the presence of oxidants, surfactants and commercial detergents. The optimum temperature, pH, incubation time and inoculum size were 55 ºC, 11, 48 h, 1 %, respectively. Calcium alginate was used as the immobilization matrix and the effects of gel concentration, bead size, age of immobilized cells, solidification period and initial biomass concentration on alkaline protease production and cell leakage were investigated. The results indicated that the immobilization was most effective with 4 % gel concentration, bead size of 3 mm, 24 h aged... Tipo: Info:eu-repo/semantics/article Palavras-chave: Optimization; Immobilized cells; Cell leakage; Detergent; Oxidant stability. Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000100017 Production of cellulase by Aspergillus niger under submerged and solid state fermentation using coir waste as a substrate BJM Mrudula,Soma; Murugammal,Rangasamy. Aspergillus niger was used for cellulase production in submerged (SmF) and solid state fermentation (SSF). The maximum production of cellulase was obtained after 72 h of incubation in SSF and 96 h in Smf. The CMCase and FPase activities recorded in SSF were 8.89 and 3.56 U per g of dry mycelial bran (DBM), respectively. Where as in Smf the CMase & FPase activities were found to be 3.29 and 2.3 U per ml culture broth, respectively. The productivity of extracellular cellulase in SSF was 14.6 fold higher than in SmF. The physical and nutritional parameters of fermentation like pH, temperature, substrate, carbon and nitrogen sources were optimized. The optimal conditions for maximum biosynthesis of cellulase by A. niger were shown to be at pH 6,... Tipo: Info:eu-repo/semantics/article Palavras-chave: Aspergillus niger; Coir waste; Cellulase; Submerged fermentation; Solid-state fermentation. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000300033 Registros recuperados: 2 Primeira ... 1 ... Última

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