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荒井, 威吉; 仙田, 晶嗣; 山上, 貴礼; 無量谷, 亜希; 中村, 正; 浦島, 匡; ARAI, lkichi; SENDA, Akitsugu; YAMAGAMI, Kihiro; MURYOYA, Aki; NAKAMURA, Tadashi; URASHIMA, Tadasu. |
The efficacy of the use of acidic electrolyzed water (AEW) for premilking teat cleaning and disinfection (TCD) was evaluated using a split-udder experimental design. The efficacy of AEW for TCD was compared with that of NaClO solution contained 200 ppm available chlorine (HClO). The viable cell counts levels of SPC,lactic acid bacteria,thermotrophilic bacteria and anaerobe (group A) which contaminated on teat skin were over 10³ cfu/cm²,whereas psychrotrophilic bacteria and coliforms (group B) were less 10² cfu/cm². After TCD with AEW and HClO,viable cell counts levels of the microorganisms of group A and group B were decreased to 2.8-7.6% and 10.0-14.5% respectively. On the other hand,the efficacy of AEW or HClO for TCD was almost constant throughout one... |
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Palavras-chave: 強酸性電解水; 乳頭清拭; Acidic electrolyzed water; Premilking teat cleaning and disinfection. |
Ano: 2002 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2993 |
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NOZAKI, Hirofumi; WIJAYAGUNAWARDANE, Missaka P.B.; KODITUWAKKU, Suranga P.; YOSHIDA, Takashi; NAKAMURA, Tadashi; ARAI, Ikichi; URASHIMA, Tadasu; MIYAMOTO, Akio; 中村, 正; 浦島, 匡; 宮本, 明夫. |
N-acetylglucosaminyltransferase I (GnT I; EC 2.4.1.101), which catalyzes the first step in the conversion of oligomannose to complex or hybrid N-glycans of glycoproteins, was found in media cultured with bovine oviduct epithelial cells (BOEC) obtained from non-pregnant cows during the follicular phase. Combined treatment with specific hormones increased GnT I release from BOEC. Luteinizing hormone (LH; 10 ng/ml) alone slightly, but together with 17β-estradiol (E2; 1 ng/ml), synergistically increased GnT I activity. Vascular endothelial growth factor (VEGF) and tumor necrosis factor (TNF) α, which have been shown to have their highest activities in the bovine oviduct during the periovulatory period, also increased in GnT I activity. This study provides the... |
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Palavras-chave: Bovine; Luteinizing hormone (LH); N-acetylglucosaminyltransferase I; Oviduct; Tumor necrosis factor (TNF) α; Vascular endothelial growth factor (VEGF). |
Ano: 2005 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/2978 |
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