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Proteomic and endophytic changes during storage of organically and conventionally grown carrots Organic Eprints
LOUARN, Sébastien; NAWROCKI, Arkadiusz; Thorup-Kristensen, Kristian; Søgaard Lund, Ole; NØRREGAARD JENSEN, Ole; Collinge, David B.; Jensen, Birgit.
This study utilises physiologically independent assays to evaluate whether there are differences in the way that organically and conventionally grown carrots develop during storage. Thus quantitative proteomics and sampling the endophytic micromycota of stored carrots provide two different assessments of the physiological state of stored carrots.
Tipo: Journal paper Palavras-chave: Vegetables; Weed management; Post harvest management and techniques; Root crops.
Ano: 2011 URL: http://orgprints.org/19485/4/19485.pdf
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Quantitative proteomics by 2DE and MALDI MS/MS uncover the effects of organic and conventional cropping methods on vegetable products Organic Eprints
NAWROCKI, Arkadiusz; Thorup-Kristensen, Kristian; Jensen, Ole Nørgaard.
Organic farming aims to be environmentally sound, but the question as to whether organic cropping brings more nutritional benefits to farmers and consumers than the conventional cropping needs still to be answered. To gain insights into the molecular effects of organic farming we used proteome analysis to analyze cabbage (Brassica oleracea L. var ‘capitata’) and carrot (Daucus carota var. ‘sativus’) Our aim was to identify the metabolic pathways that are affected by different cropping regimes and thus, may have an effect on quality, storability and pathogen resistance of crops. By means of two dimensional gel electrophoresis and MALDI tandem mass spectrometry we compared proteomes of cabbage and carrot root, obtained in the first growth season, cropped...
Tipo: Journal paper Palavras-chave: Production systems; Crop combinations and interactions; Vegetables.
Ano: 2011 URL: http://orgprints.org/19595/1/Norgaard_et_al_JPROT%2DS%2D10%2D00154%2D2.pdf
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The influence of the fungal pathogen Mycocentrospora acerina on the proteome and polyacetylenes and 6- methoxymellein in organic and conventionally cultivated carrots (Daucus carota) during post harvest storage Organic Eprints
LOUARN, Sébastien; NAWROCKI, Arkadiusz; Edelenbos, Merete; JENSEN, Dan Funck; NØRREGAARD JENSEN, Ole; Collinge, David B.; Jensen, Birgit.
Carrots may have to be discarded during post harvest cold storage due to development of fungal infections, caused by, e.g., liquorice rot caused by Mycocentrospora acerina. Control of this disease is mainly based on cultural practice. We compared the susceptibility of carrots grown under conventional and organic agricultural practices. We have developed a bioassay for infection studies of M. acerina on carrots and observed that organic roots were more susceptible after one month storage than conventional ones but no differences were apparent after four or six months storage. Levels of three polyacetylenes (falcarinol, falcarindiol and falcarindiol-3-acetate) and the isocoumarin phytoalexin (6-methoxymellein) were analysed. No changes in individual and...
Tipo: Journal paper Palavras-chave: Vegetables Crop health; Quality; Protection Post harvest management and techniques Root crops.
Ano: 2011 URL: http://orgprints.org/19471/4/19471.pdf
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Proteomic analysis of the Mycocentrospora acerina-carrot interaction during storage Organic Eprints
LOUARN, Sébastien; Jensen, Birgit; NAWROCKI, Arkadiusz; NØRREGAARD JENSEN, Ole.
During post harvest storage, a large proportion of carrots (more than 50%) may have to be discarded due to the development of liquorice rot caused by Mycocentrospora acerina. This fungus is soil borne and brought into the store in to soil adhering to the root. Liquorice rot development is mainly related to physiological or structural resistance of carrot, therefore the control of this storage disease is based on cultural practices and storage conditions. It is believed that carrots at the beginning of storage can resist disease developments due to chemical defence mechanisms involving some proteins, peptides and secondary metabolites. The hypothesis is that proteome changes during storage of carrots are related to the susceptibility to M. acerina. During...
Tipo: Conference paper, poster, etc. Palavras-chave: Crop health; Quality; Protection Post harvest management and techniques Vegetables Root crops.
Ano: 2008 URL: http://orgprints.org/16216/2/16216.pdf
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