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| Melo,Poliana de Castro; Ferreira,Luciano Menezes; Nader Filho,Antônio; Zafalon,Luiz Francisco; Vicente,Hinig Isa Godoy; Souza,Viviane de. |
| Biofilm formation is considered to be a selective advantage for Staphylococcus aureus mastitis isolates by facilitating bacterial persistence in the udder. It requires attachment to mammary epithelium, proliferation and accumulation of cells in multilayers. The objective of this study was to determine the sensitivity and specificity of three techniques for the detection of S. aureus biofilm-positive strains. Two phenotypic tests, including growth on microtitre plates and Congo red agar, were compared with a PCR technique using 94 S. aureus strains obtained from cows with subclinical mastitis from two farms in the state of São Paulo. These strains were characterised by in vitro slime production on Congo red agar, biofilm formation on microtitre plates and... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Biofilms; Bovine mastitis; Phenotypic and molecular analysis; Staphylococcus aureus. |
| Ano: 2013 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822013000100017 |
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