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| Kempf, Isabelle; Kerouanton, Annaëlle; Bougeard, Stéphanie; Nagard, Bérengère; Rose, Valérie; Mourand, Gwénaëlle; Österberg, Julia; Bengtsson, Björn O.. |
| The purpose of the study was to evaluate and compare the prevalence and antimicrobial resistance of Campylobacter coli in conventional and organic pigs from France and Sweden. Fecal or colon samples were collected at farms or at slaughterhouses and cultured for Campylobacter. The minimum inhibitory concentrations of ciprofloxacin, nalidixic acid, streptomycin, tetracycline, erythromycin, and gentamicin were determined by microdilution for a total of 263 French strains from 114 pigs from 50 different farms and 82 Swedish strains from 144 pigs from 54 different farms. Erythromycin resistant isolates were examined for presence of the emerging rRNA methylase erm(B) gene. The study showed that within the colon samples obtained in each country there was no... |
| Tipo: Journal paper |
Palavras-chave: Food security; Food quality and human health Health and welfare. |
| Ano: 2017 |
URL: http://orgprints.org/32150/1/fmicb-08-00955%20%281%29.pdf |
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| Kerouanton, Annaëlle; Nagard, Bérengère; Rose, Valérie; Samson, Vivien; Denis, Martine. |
| In the frame of the CORE Organic II funded European project SafeOrganic, fecal samples of 58 conventional pigs and 56 organic pigs, originated from 31 organic herds and 31 conventional herds, were collected in a slaughterhouse in order to isolate Campylobacter coli. Direct streaking from feces and incubation at 37°C of the Karmali plates allowed the isolation of another Campylobacter species: Campylobacter lanienae. Indeed, among the 381 typical Campylobacter colonies isolated, it was not possible to identify the species for 118 isolates with the Wang’s multiplex-PCR. However, 85 of these isolates were confirmed C. lanienae by Maldi-Tof and by 16S rRNA PCR. With the two species, coli and lanienae, the occurrence of Campylobacter in pig was... |
| Tipo: Conference paper, poster, etc. |
Palavras-chave: Food security; Food quality and human health France. |
| Ano: 2017 |
URL: http://orgprints.org/32149/2/abstract_302_A_Kerouanton.pdf |
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| Denis, Martine; Nagard, Bérengère; Rose, Valérie; Bourgoin, Kévin; Cutimbo, Mélina; Kerouanton, Annaëlle. |
| To evaluate the impact of pig farm management on the genetic diversity and on the virulence of Campylobacter coli, we characterized isolates from 19 organic pig farms (62 isolates) and from 24 conventional pig farms (58 isolates). The 120 C. coli isolates were typed using pulsed field gel electrophoresis (PFGE) and multilocus sequence typing (MLST) and the presence of nine virulence genes was screened using real-time PCR. The capacity of adhesion and invasion of 61 isolates (32 from organic and 29 from conventional farms)were then tested on human intestinal Caco-2 cells. A total of 59 PFGE types and of 50 sequence types (STs) were identified. Twelve PFGE types and nine STs, accounting for 34 and 41.6%of the isolates, respectively, were common between the... |
| Tipo: Journal paper |
Palavras-chave: Food security; Food quality and human health. |
| Ano: 2017 |
URL: http://orgprints.org/32151/1/fmicb-08-01016%20%28DENIS%29.pdf |
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