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Influence of Nutrient Stress on the Relationships between PAM Measurements and Carbon Incorporation in Four Phytoplankton Species ArchiMer
Napoleon, Camille; Raimbault, Virginie; Claquin, Pascal.
Two methods of measuring primary production, modulated fluorimetry (PAM) and the traditional carbon incorporation method (C-13), were compared in four phytoplankton species, two diatoms (Pseudo-nitzschia pungens and Asterionellopsis glacialis), and two dinoflagellates (Heterocapsa sp and Karenia mikimotoi), under N (nitrogen), P (phosphorus) and Si (silicon) limited semi-continuous culture. N and Si-limited cultures showed relatively high quantum efficiency of the PSII (F-v/F-m) values, confirming that F-v/F-m is not a good proxy for nutrient stress in balanced systems, whereas P limitation had a drastic effect on many physiological parameters. In all species, the physiological capacity of phytoplankton cells to acclimate to nutrient limitations led to...
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Ano: 2013 URL: http://archimer.ifremer.fr/doc/00152/26307/24383.pdf
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Multi-Parametric Relationships between PAM Measurements and Carbon Incorporation, an In Situ Approach ArchiMer
Napoleon, Camille; Claquin, Pascal.
Primary production (PP) in the English Channel was measured using C-13 uptake and compared to the electron transport rate (ETR) measured using PAM (pulse amplitude modulated fluorometer). The relationship between carbon incorporation (P-obs) and ETR was not linear but logarithmic. This result can be explained by alternative electron sinks at high irradiance which protect the phytoplankton from photoinhibition. A multi-parametric model was developed to estimate PP by ETR. This approach highlighted the importance of taking physicochemical parameters like incident light and nutrient concentrations into account. The variation in the ETR/P-obs ratio as a function of the light revealed different trends which were characterized by three parameters (R-max, the...
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Ano: 2012 URL: http://archimer.ifremer.fr/doc/00089/20067/17705.pdf
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Dynamics of phytoplankton diversity structure and primary productivity in the English Channel ArchiMer
Napoleon, Camille; Fiant, Liliane; Raimbault, Virginie; Riou, Philippe; Claquin, Pascal.
The dynamics of the phytoplankton assemblage, the physical, chemical and biological parameters, and primary productivity and production were monitored in the central English Channel along a transect between Ouistreham and Portsmouth from January to December 2010. The spatial patterns of the phytoplankton assemblage were controlled by the hydrological characteristics of the water masses, and the annual structure of the phytoplankton assemblage was characteristic of the central English Channel and was controlled by seasonality. The spring bloom was dominated by a single species, Chaetoceros socialis, and associated with low microphytoplankton evenness and Shannon-Wiener indices, whereas the evenness index was high from late spring to winter and associated...
Tipo: Text Palavras-chave: Phytoplankton diversity; Primary production; Productivity; English Channel.
Ano: 2014 URL: https://archimer.ifremer.fr/doc/00198/30964/29336.pdf
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