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Production of α-amylase from Streptomyces sp. SLBA-08 strain using agro-industrial by-products BABT
Santos,Édilla Ribeiro dos; Teles,Zozilene Nascimento Santos; Campos,Núria Mariana; Souza,Diogo Angeli Jacinto de; Bispo,Aline Simões da Rocha; Nascimento,Rodrigo Pires do.
Approximately 1.5 trillion tons are the estimated yearly biomass production, making it an essentially unlimited source of raw material for environmentally friendly and biocompatible products transformed by microorganism, specially fungi and actinomycetes. Several lignocellulosic residues, such as sisal waste and sugarcane bagasse contain starch in their structures which could become important sources for the production of amylases. This study evaluated the production of amylolytic enzymes using Streptomyces sp. SLBA-08 strain, isolated from a semi-arid soil, according to their ability to grow on soluble starch as the sole carbon source. The effect of the carbon source (sisal waste and sugarcane bagasse) on α-amylase production was studied using submerged...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Α-amylases; Sugarcane bagasse; Sisal waste; Streptomyces sp.
Ano: 2012 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132012000500020
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Brewer's spent grain and corn steep liquor as alternative culture medium substrates for proteinase production by Streptomyces malaysiensis AMT-3 BJM
Nascimento,Rodrigo Pires do; Alves Junior,Nelson; Coelho,Rosalie Reed Rodrigues.
Brewer's spent grain and corn steep liquor or yeast extract were used as the sole organic forms for proteinase production by Streptomyces malaysiensis in submerged fermentation. The influence of the C and N concentrations, as well as the incubation periods, were assessed. Eight proteolytic bands were detected through gelatin-gel-electrophoresis in the various extracts obtained from the different media and after different incubation periods, with apparent molecular masses of 20, 35, 43, 50, 70, 100, 116 and 212 kDa. The results obtained suggest an opportunity for exploring this alternative strategy for proteinases production by actinomycetes, using BSG and CSL as economically feasible substrates.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Streptomyces malaysiensis; Brewer's spent grains; Corn steep liquor; Proteinases; Submerged fermentation.
Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822011000400020
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