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	Provedor de dados Ciênc. Tecnol. Aliment. (1) Autor Herculano,Polyanna Nunes (1) Moreira,Keila Aparecida (1) Nascimento,Talita Camila Evaristo da Silva (1) Silva,Anna Carolina da (1) Silva,Sheylla Araújo da (1) Palavra-chave Plant proteases (1) Proteolytic activity (1) Rennet (1) Tipo do documento Info:eu-repo/semantics/article (1) Ano 2013 (1) País Brazil (1) Idioma Inglês (1)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 1 Primeira ... 1 ... Última Potential of quixaba (Sideroxylon obtusifolium) latex as a milk-clotting agent Ciênc. Tecnol. Aliment. Silva,Anna Carolina da; Nascimento,Talita Camila Evaristo da Silva; Silva,Sheylla Araújo da; Herculano,Polyanna Nunes; Moreira,Keila Aparecida. There are several obstacles to the use of chymosin in cheese production. Consequently, plant proteases have been studied as possible rennet substitutes, but most of these enzymes are unsuitable for the manufacture of cheese. The aim of this study was to evaluate the potential of latex from Sideroxylon obtusifolium as a source of milk-clotting proteases and to partially characterize the enzyme. The enzyme extract showed high protease and coagulant activities, with an optimal pH of 8.0 and temperature of 55 °C. The enzyme was stable in wide ranges of temperature and pH. Its activity was not affected by any metal ions tested; but was inhibited by phenylmethanesulfonyl fluoride and pepstatin. For the coagulant activity, the optimal concentration of CaCl2 was... Tipo: Info:eu-repo/semantics/article Palavras-chave: Proteolytic activity; Plant proteases; Rennet. Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000300017 Registros recuperados: 1 Primeira ... 1 ... Última

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