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| Calado,Daniéla; Navarro-Silva,Mario Antonio; Sallum,Maria Anice Mureb. |
| The aim of the present study was to examine genetic variability in populations of An. cruzii by employing PCR-RAPD and PCR-RFLP markers. All analyses were carried out using individuals of the F1 generation of wild caught females obtained in Santa Catarina State (Florianópolis and São Francisco do Sul), Paraná State (Morretes, Paranaguá and Guaratuba) and São Paulo State (Cananéia). In the PCR-RAPD experiments, seven primers were used for comparisons within and among populations. The restriction profile of the ITS2 including a fragment of both 5.8S and 28S regions of the rDNA was obtained with the enzymes BstUI, HaeIII, TaqI, HhaI, Sau96I, HinfI, HincII and NruI. The PCR-RAPD method detected a large number of polymorphic bands. Genetic distance among... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Anophelinae; Genetic variability; ITS2; Molecular markers. |
| Ano: 2006 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262006000300014 |
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| Calado,Daniéla C.; Navarro-Silva,Mario Antonio. |
| Influence of temperature on longevity, fecundity, and haematophagic activity of Aedes (Stegomyia) albopictus Skuse, 1894 (Diptera, Culicidae) under laboratory condition. Based on adults of Aedes albopictus maintained under four constant temperatures, 15ºC, 20ºC, 25ºC and 30ºC, longevity of males and females, fecundity, eggs viability, number of days from emergence to the first bloodsucking, and duration of the preoviposition period were observed. The average of the longevity for female was 19.45, 59.35, 61.55 and 42.72 days, under 15ºC, 20ºC, 25ºC and 30ºC, respectively. The greatest average number of bloodsucking per female was 8.32, under 25ºC. The average number of eggs per female was 1.00, 73.91, 269.87 and 183.69, and the greatest rates of viable eggs... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Aedes albopictus; Biology; Fecundity; Longevity; Temperature. |
| Ano: 2002 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0085-56262002000100011 |
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| Navarro-Silva,Mario Antonio; Barbosa,Andreia Aparecida; Calado,Daniéla. |
| Fragmentos de mata no cenário urbano podem reunir condições favoráveis à reprodução de espécies de Culicidae. No Parque Regional do Iguaçu (Curitiba, Paraná) existe uma série de cavas ou lagoas contendo vegetação aquática que pode ser utilizada pelos imaturos das espécies de Mansoniini. A investigação foi desenvolvida com objetivo de conhecer as espécies de Mansonia Blanchard, 1901 que exercem hematofagia no período diurno em área de mata no interior do parque. As coletas dos mosquitos foram efetuadas de setembro de 1999 a agosto de 2000. Três espécies de Mansonia foram identificadas, com os seguintes percentuais relativos: 74,7% de M. fonsecai (Pinto, 1932), 10,7% de M. wilsoni (Barreto & Coutinho, 1944) e 8,3% de M. tittilans (Walker, 1848).... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Mansonia wilsoni; Mansonia tittilans; Mansonia fonsecai; Vetores. |
| Ano: 2004 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752004000200012 |
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| Barbosa,Andréia Aparecida; Navarro-Silva,Mario Antonio; Calado,Daniéla. |
| Vegetation islands inside urban area can propitiate the formation of: a) adults refuge to many mosquitoes species, b) diversity of hosters, c) allows the proliferation of immature stages through naturals and artificial breeding, these ones were done by the interaction between antropic activity developed in these spaces or around areas. Considering the possibilities of contacts between the vector and the human population, the aim of this work was to verify the Culicidae species that can use man as blood source in the restricted areas of Botanical Garden, Curitiba. The capture of adults using the method of human bait at soil level, inside the forest, monthly during the period of October 13th 1998 to September 22nd 1999, from 5:00 p.m. to 8:00 p.m. 312... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Antropic environment; Culicidae; Human bait. |
| Ano: 2003 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-81752003000100007 |
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