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Preparation and characterization of κ-carrageenase immobilized onto magnetic iron oxide nanoparticles Electron. J. Biotechnol.
Xiao,Anfeng; Xu,Caiyun; Lin,Yan; Ni,Hui; Zhu,Yanbing; Cai,Huinong.
Background Carboxyl-functionalized magnetic nanoparticles were synthesized via chemical co-precipitation method and modified with oleic acid which was oxidized by potassium permanganate, and κ-carrageenase from Pseudoalteromonas sp. ASY5 was subsequently immobilized onto them. The immobilization conditions were further optimized, and the characterizations of the immobilized κ-carrageenase were investigated. Results The κ-carrageenase was immobilized onto magnetic iron oxide nanoparticles, and the bonding was verified by Fourier transform infrared spectroscopy. The optimal conditions for κ-carrageenase immobilization were 2.5% (w/v) glutaraldehyde, 13.9 U κ-carrageenase for 20 mg of magnetic nanoparticles, a 2-h...
Tipo: Journal article Palavras-chave: Carrageenase; Characterization; Immobilization; Nanoparticles.
Ano: 2016 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582016000100001
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Statistical optimization for tannase production by Aspergillus tubingensis in solid-state fermentation using tea stalks Electron. J. Biotechnol.
Xiao,Anfeng; Huang,Yufeng; Ni,Hui; Cai,Huinong; Yang,Qiuming.
Background A sequential statistical strategy was used to optimize tannase production from Aspergillus tubingensis using tea stalks by solid-state fermentation. Results First, using a Plackett-Burman design, inoculum size and incubation time (among seven tested variables) were identified as the most significant factors for tannase yield. The effects of significant variables were further evaluated through a single steepest ascent experiment and central composite design with response surface analysis. Under optimal conditions, the experimental value of 84.24 units per gram of dry substrate (U/gds) closely matched the predicted value of 87.26 U/gds. Conclusions The result of the statistical approach was 2.09 times higher than the basal medium (40.22 U/gds)....
Tipo: Journal article Palavras-chave: Aspergillus tubingensis; Process optimization; Response surface methodology; Tea stalks; Tannase fermentation.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300001
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Study on the relationship between intracellular metabolites and astaxanthin accumulation during Phaffia rhodozyma fermentation Electron. J. Biotechnol.
Xiao,Anfeng; Jiang,Xinglong; Ni,Hui; Yang,Qiuming; Cai,Huinong.
Background To study the relationship between intracellular anabolism and astaxanthin production, the influence of intracellular protein and fatty acids on astaxanthin production by four mutant Phaffia rhodozyma strains and their variations was investigated in this research. Results First, the content of astaxanthin in cells showed a reverse fluctuation in contrast to that of protein during the whole fermentation process. Moreover, compared with the three other strains, the astaxanthin-overproducing mutant strain of the yeast P. rhodozyma, called JMU-MVP14, had the highest specific productivity of astaxanthin as 6.8 mg/g, whereas its intracellular protein and fatty acid contents were the lowest. In addition, as a kind of sugar metabolic product, ethanol was...
Tipo: Journal article Palavras-chave: Astaxanthin; Ethanol; Fatty acids; Phaffia rhodozyma; Protein.
Ano: 2015 URL: http://www.scielo.cl/scielo.php?script=sci_arttext&pid=S0717-34582015000300002
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