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Methodenentwicklung zum quantitativen und qualitativen Nachweis von Brandkrankheiten (Tilletia spp.) bei Weizen mittels quantitativer Real-Time PCR (qPCR) Organic Eprints
Grundler, MK; Sedlmeier, Monika; Killermann, Berta; Niessen, L..
For a precise diagnosis of the wheat bunt diseases, common bunt and dwarf bunt (Tilletia spp.), reliable and reproducible methods are essential. Further the accurate quantification of the spore number on infested seed is important for seed multiplication and international seed trading, as different thresholds and quarantine regulations have to be considered. At present the determination of the teliospores is carried out according to a filtration method (ISTA 1984) combined with a microscopic examination requiring considerable experience to distinguish the different Tilletia species. Therefore the aim of this research work is to develop an efficient molecular testing method by means of quantitative Real-time PCR (qPCR). Based on genome data species...
Tipo: Conference paper, poster, etc. Palavras-chave: Cereals; Pulses and oilseeds Crop health; Quality; Protection.
Ano: 2017 URL: http://orgprints.org/31621/1/Methodenentwicklung%20zum%20quantitativen%20und%20qualitativen.pdf
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