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Kinetics and Thermodynamics of Thermal Inactivation of β-Galactosidase from Aspergillus oryzae BABT
Klein,Manuela Poletto; Sant’Ana,Voltaire; Hertz,Plinho Francisco; Rodrigues,Rafael Costa; Ninow,Jorge Luiz.
ABSTRACT For optimization of biochemical processes in food and pharmaceutical industries, the evaluation of enzyme inactivation kinetic models is necessary to allow their adequate use. Kinetic studies of thermal inactivation of β-galactosidase from Aspergillus oryzae were conducted in order to critically evaluate mathematical equations presented in the literature. Statistical analysis showed that Weibull model presented the best adequacy to residual enzymatic activity data through the processing time and its kinetic parameters as a function of the temperature, in the range of 58-66 ºC. The investigation suggests the existence of a non-sensitive heat fraction on the enzyme structure, which is relatively stable up to temperatures close to 59 ºC....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Β-Galactosidase; Enzyme Inactivation; Modelling; Protein Denaturation; Weibull.
Ano: 2018 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132018000100409
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Development of an alternative technology for the oyster mushroom production using liquid inoculum Ciênc. Tecnol. Aliment.
Silveira,Márcia Luciane Lange; Furlan,Sandra Aparecida; Ninow,Jorge Luiz.
Pleurotus ostreatus, worldwide known as oyster mushroom, was cultivated in banana straw using inocula produced by two different processes - liquid inoculum and the traditionally used solid inoculum. Different ratios (5, 10, 15, and 20%) were tested. Biological efficiency, yield, productivity, organic matter loss, and moisture of fruiting bodies as well as physical-chemical characteristics of banana straw were studied. Significant differences were observed for cellulose, lignin, and hemicellulose content between one and two harvests for both solid and liquid inocula. It was observed that P. ostreatus growth promoted higher degradation of lignin (80.36%), followed by hemicellulose (78.64%) and cellulose (60.37%). Significant differences between one and two...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Liquid inoculum; Solid inoculum; Inoculation ratio.
Ano: 2008 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612008000400014
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Monascus pigment production in bioreactor using a co-product of biodiesel as substrate Ciênc. Tecnol. Aliment.
Bühler,Rose Marie Meinicke; Dutra,Anderson Cesar; Vendruscolo,Francielo; Moritz,Denise Esteves; Ninow,Jorge Luiz.
The study and use of natural pigments in food industries have increased in recent years due to the toxicity presented by artificial pigments. Monascus ruber is a filamentous fungus that produces red, orange, and yellow pigments under different growing conditions. The growth of health food market has increased in parallel with the growth in biofuels production, such as biodiesel, which generates a concomitant increase in the production of glycerin that can be used in bioprocesses. The objective of this study was to use glycerin and glucose as substrates in the production of natural pigments in a bioreactor. The culture of Monascus ruber was carried out in a Bioflo III reactor with 4 L of working volume and pH, temperature, aeration, and agitation control....
Tipo: Info:eu-repo/semantics/article Palavras-chave: Monascus ruber; Glycerin; Bioreactor.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000500002
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