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| Cerqueira Melo, Rita de Cássia; Dorea Bragança, Carlos Augusto; Nogueira Pestana, Katia; Silva, Harllen Sandro Alves da; Ferreira, Claudia Fortes; Alves Santos de Oliveira, Saulo. |
| Modifications were made in the PCR conditions aiming to overcome the problem of non-amplification of the Xanthomonas phaseoli pv. manihotis (Xpm) fragment, using the primer pair XV / XK described in the literature. The objective of this study was to propose changes in the primers already described (XV / XK_MOD) and validate the use of these new primers in identifying Xpm. The validation procedure was carried out with the primer pair XV and XK_MOD, using different strains of Xpm, other plant pathogenic and endophytic bacteria genera and one isolate of X. phaseoli pv. passiflorae. As a control, additional reactions were conducted in multiplex with the universal primers for the 16S rRNA gene of the bacteria together with XV / XK and XV / XK_MOD. Using the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Xpm; Cassava bacterial blight; Molecular diagnosis; Resistance. |
| Ano: 2019 |
URL: http://periodicos.uem.br/ojs/index.php/ActaSciAgron/article/view/42708 |
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