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Pereira,M.A.A.; Nowosh,V.; Suffys,P.N.; Queiroz,G.B.; Silva,K.M.O.; Lourenço,M.C.S.; Vicente,A.C.P.; Fontes,A.N.B.; Morgado,S.; Neves,R.C.S.M.. |
ABSTRACT Canine Leproid Granuloma Syndrome (CLGS), also known as canine leprosy, is a cutaneous nodular infectious disease caused by Mycobacterium sp.. Despite being reported worldwide, it is still quite unknown and underdiagnosed. Diagnosis may be achieved by cytopathology or histopathology of skin lesions, but identification of the infectious agent is complex, since bacterial in vitro growth is not possible, relying upon molecular techniques such as PCR to confirm Mycobacterium DNA in the sample. We report a CLGS case in Niteroi, Rio de Janeiro state, Brazil, diagnosed by cytopathology and submitted to molecular identification of the agent. PCR amplification of hsp65 gene was performed and revealed 100% genetic homology to M. murphy strain. This is the... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: CLGS; Hsp65 sequence; Isolate Metcalfe; Molecular methods. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0102-09352018000601699 |
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