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Use of oval conidia as a tool to assess the genetic transfer among Colletotrichum sublineolum mutants BABT
Nunes,Maria Paula; Souza-Paccola,Ednéia Aparecida de; Andrade-Nóbrega,Gisele Maria; Paccola-Meirelles,Luzia Doretto.
The aim of this study was to assess oval conidia production in mutants for the reductase nitrate gene in C. sublineolum and verify the possibility of using them as a tool to transfer the genetic material in this species. The mutants used in the present study lost the ability to form falcate conidia but all produced oval conidia. The number of nuclei by conidium was evaluated. Oval conidia were efficient in the heterokaryon formation and these heterokaryons in liquid culture medium they also produced oval conidia in abundance. Recombinants were obtained and the genetic exchanges were confirmed by the RAPD analyses.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Genetic recombination; Nit mutants; Parameiosis.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132010000100021
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