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| YBAÑEZ, Adrian Patalinghug; SATO, Fumio; NAMBO, Yasuo; FUKUI, Takashi; MASUZAWA, Toshiyuki; OHASHI, Norio; MATSUMOTO, Kotaro; KISHIMOTO, Toshio; INOKUMA, Hisashi; 松本, 高太郎; 猪熊, 壽. |
| A total of 87 Thoroughbred horses and 10 ixodid ticks from a ranch in Hidaka district, Hokkaido were tested for tick-borne diseases. Using the indirect fluorescent antibody (IFA) method, 3.4, 92.0 and 97.7% of the horses showed antibody titers of ≥80 against Anaplasma phagocytophilum, Rickettsia helvetica, and Borrelia garinii, respectively. This is the first report of infection with the 3 pathogens in horses in Japan. Using PCR, DNAs from the peripheral blood of all horses were found negative with any Anaplasma, Rickettsia and Borrelia spp., while those from Haemaphysalis megaspinosa ticks were found positive for Anaplasma sp. closely related to A. phagocytophilum in Japan, and A. bovis. B. japonica was also detected in an H. flava tick for the first time. |
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Palavras-chave: Anaplasma sp.; Borrelia sp.; Rickettsia sp.; Thoroughbred horse; Tick-borne diseases. |
| Ano: 2013 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3840 |
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| MATSUMOTO, Kotaro; TAKEUCHI, Toshihiko; YOKOYAMA, Naoaki; KATAGIRI, Yoshito; OOSHIRO, Mamoru; ZAKIMI, Satoshi; Gaowa; KAWAMORI, Fumihiko; OHASHI, Norio; INOKUMA, Hisashi. |
| We collected a total of 206 Haemaphysalis longicornis ticks by flagging in pastures in Yonaguni Island, Okinawa, Japan, in April 2008. Four of the 206 tick DNA samples tested were positive in a polymerase chain reaction (PCR) screening for the 16SrRNA gene of Anaplasmataceae. Partial sequences of 4 PCR products were identical to each other. Longer sequences of the 16SrRNA gene were successfully determined in 2 of the 4 tick samples, and the obtained 1,392 bp and 1,300 bp sequences revealed high similarity to the 16SrRNA gene sequences of the validated Ehrlichia species, including Ehrlichia ewingii, E. chaffeensis, and E. canis (98.3–98.6%). We also sequenced 1,304 bp of the groEL gene from the 2 tick samples, and found that these had the highest similarity... |
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Palavras-chave: 16SrRNA; Ehrlichia; GroEL; Haemaphysalis longicornis. |
| Ano: 2011 |
URL: http://ir.obihiro.ac.jp/dspace/handle/10322/3581 |
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