|
|
|
|
|
| Vannier, Pauline; Marteinsson, Viggo Thor; Fridjonsson, Olafur Hedinn; Oger, Philippe; Jebbar, Mohamed. |
| Thermococcus barophilus is a hyperthermophilic, anaerobic, mixed heterotrophic, and carboxydotrophic euryarchaeon isolated from the deep sea hydrothermal vent Snakepit site on the mid-Atlantic ridge at a depth of 3,550 m. T. barophilus is the first true piezophilic, hyperthermophilic archaeon isolated, having an optimal growth at 40 MPa. Here we report the complete genome sequence of strain MP, the type strain of T. barophilus. The genome data reveal a close proximity with Thermococcus sibiricus, another Thermococcus isolated from the deep biosphere and a possible connection to life in the depths. |
| Tipo: Text |
|
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00031/14185/11865.pdf |
| |
|
|
| Golub, Maksym; Martinez, Nicolas; Michoud, Gregoire; Ollivier, Jacques; Jebbar, Mohamed; Oger, Philippe; Peters, Judith. |
| In live cells, high concentrations up to 300–400 mg/mL, as in Eschericia coli (Ellis, R. J. Curr. Opin. Struct. Biol. 2001, 11, 114) are achieved which have effects on their proper functioning. However, in many experiments only individual parts of the cells as proteins or membranes are studied in order to get insight into these specific components and to avoid the high complexity of whole cells, neglecting by the way the influence of crowding. In the present study, we investigated cells of the order of Thermococcales, which are known to live under extreme conditions, in their intact form and after cell lysis to extract the effect of crowding on the molecular dynamics of the proteome and of water molecules. We found that some parameters characterizing the... |
| Tipo: Text |
|
| Ano: 2018 |
URL: https://archimer.ifremer.fr/doc/00454/56526/58329.pdf |
| |
|
|
| Jun, Xu; Liu Lupeng,; Xu Minjuan,; Oger, Philippe; Wang Fengping,; Jebbar, Mohamed; Xiang, Xiao. |
| Pyrococcus yayanosii CH1 is the first obligate piezophilic hyperthermophilic archaeon isolated from the deep-sea hydrothermal site Ashadze on the mid-Atlantic ridge at a depth of 4,100 m. This organism grows within a temperature range of 80 to 108 degrees C and a hydrostatic pressure range of 20 to 120 MPa, with optima at 98 degrees C and 52 MPa, respectively. Here, we report the complete genome sequence (1,716,817 bp, with a G+C content of 51.6%) of the type strain P. yayanosii CH1(T) (= JCM 16557). This genomic information reveals a systematic view of the piezoadaptation strategy and evolution scenario of metabolic pathways in Thermococcales. |
| Tipo: Text |
|
| Ano: 2011 |
URL: http://archimer.ifremer.fr/doc/00041/15247/12621.pdf |
| |
|
|
| Dalmasso, Cecile; Oger, Philippe; Selva, Gwendoline; Courtine, Damien; L'Haridon, Stephane; Garlaschelli, Alexandre; Roussel, Erwan; Miyazaki, Junichi; Reveillaud, Julie; Jebbar, Mohamed; Takai, Ken; Maignien, Lois; Alain, Karine. |
| A novel strictly anaerobic, hyperthermophilic archaeon, designated strain CDGST, was isolated from a deep-sea hydrothermal vent in the Cayman Trough at 4964 m water depth. The novel isolate is obligate anaerobe and grows chemoorganoheterotrophically with stimulation of growth by sulphur containing compounds. Its growth is optimal at 75 °C, pH 6.0 and under a pressure of 50 MPa. It possesses the broadest hydrostatic pressure range for growth that has ever been described for a microorganism. Its genomic DNA G + C content is 51.11 mol%. The novel isolate belongs to the genus Thermococcus. Phylogenetic analyses indicated that it is most closely related to Thermococcus barossii DSM17882T based on its 16S rRNA gene sequence, and to ‘Thermococcus onnurineus’ NA1... |
| Tipo: Text |
Palavras-chave: Thermococcus; Piezophile; Hydrothermal vent; Cayman Trough. |
| Ano: 2016 |
URL: http://archimer.ifremer.fr/doc/00348/45949/45655.pdf |
| |
|
|
| Zeng, Xiang; Birrien, Jean-louis; Fouquet, Yves; Cherkashov, Georgy; Jebbar, Mohamed; Querellou, Joel; Oger, Philippe; Cambon-bonavita, Marie-anne; Xiao, Xiang; Prieur, Daniel. |
| A novel hydrothermal site was discovered in March 2007, on the mid-Atlantic ridge during the cruise 'Serpentine'. At a depth of 4100 m, the site 'Ashadze' is the deepest vent field known so far. Smoker samples were collected with the ROV 'Victor 6000' and processed in the laboratory for the enrichment of anaerobic heterotrophic microorganisms under high-temperature and high-hydrostatic pressure conditions. Strain CH1 was successfully isolated and assigned to the genus Pyrococcus, within the Euryarchaeota lineage within the Archaea domain. This organism grows within a temperature range of 80 to 108 degrees C and a pressure range of 20 to 120 MPa, with optima for 98 degrees C and 52 MPa respectively. Pyrococcus CH1 represents the first obligate piezophilic... |
| Tipo: Text |
Palavras-chave: Thermococcales; Extremophile; Deep sea hydrothermal vent; High hydrostatic pressure; Archaea. |
| Ano: 2009 |
URL: http://archimer.ifremer.fr/doc/2009/publication-6812.pdf |
| |
|
|
| Peters, Judith; Martinez, Nicolas; Michoud, Gregoire; Carlo, Anais; Franzetti, Bruno; Oger, Philippe; Jebbar, Mohamed. |
| The majority of the biosphere is a high pressure environment. Around 70% of the marine biosphere lies at depths below 1000 m, i.e. at pressures of 100 bars or higher. To survive in these environments, deep-biosphere organisms have adapted to life at high pressure. In vitro studies showed that the activity of certain proteins originating from deep-sea organisms is less affected by high pressure than that of enzymes from surface organisms [1-3]. However, the genetic and structural bases for this increased pressure resistance are still unknown. Elastic incoherent neutron scattering studies, which provide access to information about molecular dynamics, constitute a very promising approach to decipher the structural adaptation in proteins living under high... |
| Tipo: Text |
Palavras-chave: Neutron Scattering; Molecular Dynamics; High Pressure; Deep Sea Microbes. |
| Ano: 2014 |
URL: http://archimer.ifremer.fr/doc/00251/36186/34725.pdf |
| |
|
|
| Dalmasso, Cécile; Oger, Philippe; Courtine, Damien; Georges, Myriam; Takai, Ken; Maignien, Lois; Alain, Karine. |
| We report the genome sequence of Thermococcus superprofundus strain CDGST, a new piezophilic and hyperthermophilic member of the order Thermococcales isolated from the world’s deepest hydrothermal vents, at the Mid-Cayman Rise. The genome is consistent with a heterotrophic, anaerobic, and piezophilic lifestyle. |
| Tipo: Text |
|
| Ano: 2016 |
URL: https://archimer.ifremer.fr/doc/00345/45616/45237.pdf |
| |
|
|
|
