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	Provedor de dados BABT (2) Autor Oliva Neto,Pedro de (2) Carvalho,Ana Flavia Azevedo (1) Lima,Fabíola Aliaga de (1) Oliveira,Antonio Martins (1) Santos,Catarina dos (1) Silva,Douglas Fernandes da (1) Silva,Ketrin Cristina da (1) Mais... Palavra-chave Autolysis (1) Ethanol (1) Lactic acid bacteria (1) Lactobacillus fermentum (1) NaCl plasmolysis (1) Nucleotides (1) RNA extraction (1) Saccharomyces cerevisiae (1) Yeast extract (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2014 (1) 2011 (1) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Improvement in RNA extraction from S. cerevisie by optimization in the autolysis and NH3 hydrolysis BABT Oliveira,Antonio Martins; Oliva Neto,Pedro de. The optimization of autolysis of Saccharomyces cerevisiae from brewery was studied aiming at the maximum ribonucleic acid extraction and yeast extract production. The best conditions for yeast autolysis was 55.2ºC, pH= 5.1 and 9.8% NaCl for 24h of processing, without the NH3 use. In these conditions, the RNA yield was 89.7%, resulting in 51.3% of dehydrated yeast extract with 57.9% protein. The use of 12.2% NH3 at 60ºC after autolysis (8h) and plasmolysis (8h) was not viable due to the reduction in the RNA yield from 89.7to78.4%. On the other hand, the thermal shock at 60ºC for 15 minutes prior to autolysis provided an increase in the yield from 89.7 to 91.4%. The autolysis, including NaCl plasmolysis in the optimized conditions was efficient, economic and... Tipo: Info:eu-repo/semantics/article Palavras-chave: Autolysis; NaCl plasmolysis; Yeast extract; RNA extraction; Nucleotides. Ano: 2011 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132011000500019 Chemical inhibition of the contaminant Lactobacillus fermentum from distilleries producing fuel bioethanol BABT Oliva Neto,Pedro de; Lima,Fabíola Aliaga de; Silva,Ketrin Cristina da; Silva,Douglas Fernandes da; Carvalho,Ana Flavia Azevedo; Santos,Catarina dos. The purpose of this study was to determine the Minimum Inhibitory Concentration (MIC) of pure or mixed chemicals for Saccharomyces cerevisiae and Lactobacillus fermentum in the samples isolated from distilleries with serious bacterial contamination problems. The biocides, which showed the best results were: 3,4,4' trichlorocarbanilide (TCC), tested at pH 4.0 (MIC = 3.12 mg/l), TCC with benzethonium chloride (CBe) at pH 6.0 (MIC = 3.12 mg/l) and TCC mixed with benzalkonium chloride (CBa) at pH 6.0 (MIC = 1.53 mg /l). If CBa was used in sugar cane milling in 1:1 ratio with TCC, a 8 times reduction of CBa was possible. This formulation also should be tested in fermentation steps since it was more difficult for the bacterium to develop resistance to biocide.... Tipo: Info:eu-repo/semantics/article Palavras-chave: Ethanol; Lactic acid bacteria; Saccharomyces cerevisiae; Lactobacillus fermentum. Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000300019 Registros recuperados: 2 Primeira ... 1 ... Última

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