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Influence of high temperature on the reproductive biology of dry edible bean (Phaseolus vulgaris L.) Scientia Agricola
Silva,Daiana Alves da; Pinto-Maglio,Cecília Alzira Ferreira; Oliveira,Érica Cristina de; Reis,Raquel Luiza de Moura dos; Carbonell,Sérgio Augusto Morais; Chiorato,Alisson Fernando.
ABSTRACT: The aim of this study was to investigate the effect of heat stress on 12 bean genotypes through the analysis of their reproductive biology in terms of flowering, pollen viability, meiotic behavior, and production. Plants were grown in a climate chamber at 25-20 °C (day and night) and at a high temperature treatment 37-26 °C (day and night) from the vegetative (V4) development stage to physiological maturity. The experimental design was 2 × 12 factorial arrangement with six replications and the factors consisted of heat treatments and genotypes. In three replications, the number of newly opened flowers was checked daily. At physiological maturity, the following traits were evaluated: percentage of pod set, number of pods, number of viable seeds,...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Common bean; Heat stress; Pollen viability; Meiosis; Seed yield.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000301100
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Cytogenetic mapping of the ALMT (aluminum-activated malate transporter) gene in wheat genotypes Scientia Agricola
Oliveira,Érica Cristina de; Pinto-Maglio,Cecília Alzira Ferreira.
ABSTRACT: Presence of the ALMT1 (aluminum-activated malate transporter) gene confers resistance to aluminum toxicity in Triticum aestivum (common wheat). No resistant cultivars of Triticum turgidum ssp. Durum Desf. (durum wheat) have been registered in Brazil. The aim of this study was to map the ALMT1 through application of the FISH (fluorescence in situ hybridization) technique in five wheat genotypes, common and durum, from the Active Germplasm Bank (AGB) of the Instituto Agronômico (IAC): BH 1146, P19, P33, Anahuac, and IAC 1003. FISH-ALMT1 signals were registered in Anahuac (sensitive) chromosomes and in BH 1146, P19, and P33 (resistant) chromosomes. In the three resistant genotypes, a characteristic double FISH signal was found, located in different...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum sp.; Molecular cytogenetics; Fluorescence in situ hybridization; Aluminum tolerance; Toxicity.
Ano: 2020 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-90162020000501102
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Cytomolecular characterization of cultivars and landraces of wheat tolerant and sensitive to aluminum toxicity Bragantia
Oliveira,Érica Cristina de; Pinto-Maglio,Cecília Alzira Ferreira.
ABSTRACT Karyotyping and chromosome complement knowledge are a prerequisite for cytogenetic mapping. The aim of this study was to characterize 1 common wheat cultivar (BH 1146) and 4 durum wheat genotypes (IAC 1003, Langdon, P19 and P33) from the breeding program of the Agronomic Institute of Campinas (IAC), using karyotype analysis together with Chromomycin A3/4’-6-Diamidino-2-phenylindole (CMA/DAPI) banding techniques for longitudinal characterization of chromosomes and fluorescence in situ hybridization (FISH) in the mapping of the 45S rDNA region. Durum wheat chromosome complement number was 2n = 4x = 28 with the following karyotype formula: 22m + 6sm. Common wheat chromosome complement number was 2n = 6x = 42 with the following karyotype formula: 34m...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Triticum aestivum; Triticum turgidum ssp. durum; Karyotype; CMA/DAPI banding; FISH-rDNA; Cytomolecular mapping.
Ano: 2017 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0006-87052017000400456
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