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	Provedor de dados BJM (2) Autor Oliveira,Anna Paula de (2) Roehe,Paulo Michel (2) Batista,Helena B. de C. Ruthner (1) Campos,Karen (1) David,Cíntia de (1) Englert,Gelsa Edith (1) Franco,Ana Cláudia (1) Hübner,Sílvia de Oliveira (1) Mergener,Michelle (1) Rijsewijk,Franciscus A.M. (1) Simonetti,Amauri Braga (1) Mais... Palavra-chave BoHV-5 (1) Bovine herpesvirus 5 (1) Cell culture (1) Contamination (1) Deletion mutant (1) Fetal bovine serum (FBS) (1) Glycoprotein genes (1) Nanobacteria (1) Mais... Tipo do documento Info:eu-repo/semantics/article (2) Ano 2007 (2) País Brazil (2) Idioma Inglês (2)		Ordenar por:  Relevância Autor Título Ano Registros recuperados: 2 Primeira ... 1 ... Última Construction and characterization of a bovine herpesvirus 5 mutant with a deletion of the gI, gE and US9 genes BJM Franco,Ana Cláudia; Hübner,Sílvia de Oliveira; Oliveira,Anna Paula de; Batista,Helena B. de C. Ruthner; Roehe,Paulo Michel; Rijsewijk,Franciscus A.M.. Bovine herpesvirus 5 (BoHV-5) is a important cause of viral encephalitis in cattle in South America. Within the framework of developing a differential vaccine against BoHV-5, a deletion mutant was constructed based on a Brazilian BoHV-5 isolate. The target of the deletions were genes that code proteins implicated in the neurovirulence of BoHV-5, the glycoprotein I (gI), glycoprotein E (gE) and membrane protein US9. To construct the deletion mutant of BoHV-5, the flanking regions of all three genes were cloned in a prokaryotic plasmid. This deletion fragment was co-transfected with the viral DNA into bovine cells. Identification of deletion mutants was performed by immunostaining with an anti-gE monoclonal antibody. One of the gE negative viral populations... Tipo: Info:eu-repo/semantics/article Palavras-chave: Bovine herpesvirus 5; BoHV-5; Deletion mutant; Glycoprotein genes. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000400016 Nanobacteria-like particles: a threat to cell cultures BJM Simonetti,Amauri Braga; Englert,Gelsa Edith; Campos,Karen; Mergener,Michelle; David,Cíntia de; Oliveira,Anna Paula de; Roehe,Paulo Michel. The main goal of this study is to alert researchers who work with cell cultures for the risk of contamination by structures called nanobacteria (NB). NB are tiny structures with size varying from 80 to 500 nm, commonly occurring in clusters and producing a biofilm which contains carbonate or hydroxyl apatite. The most likely source of cell culture contamination by such organisms is serum used as supplement in culture media. The presence of NB leads to a progressive culture deterioration with accumulation of granules (probably phagocytized NB) in cytoplasmic vacuoles, an increasing number of dead cells in the supernatant and degeneration of cells that remained attached to the bottom of the vessel. NB can also be found in culture supernatants where they are... Tipo: Info:eu-repo/semantics/article Palavras-chave: Nanobacteria; Cell culture; Fetal bovine serum (FBS); Contamination. Ano: 2007 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822007000100032 Registros recuperados: 2 Primeira ... 1 ... Última

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