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Production and characterization of an extracellular lipase from Candida guilliermondii BJM
Oliveira,Anne Caroline Defranceschi; Fernandes,Maria Luiza; Mariano,André Bellin.
Extracellular lipases from the endophytic yeast Candida guilliermondii isolated from castor leaves (Ricinus communis L.) were produced using low-cost raw materials such as agro-industrial residues and applying them in the esterification of oleic acid for evaluating their potential use in biodiesel production. After partial purification using ammonium sulfate, the enzyme was characterized and presented higher activity (26.8 ± 1.5 U mL-1) in the presence of 5 mmol L-1 NaCl at 30 ºC and pH 6.5. The production through submerged fermentation was formerly performed in 150 mL erlenmeyer flasks and, once the enzyme production was verified, assays in a 14 L bioreactor were conducted, obtaining 18 ± 1.4 U mL-1. The produced enzyme was applied in the oleic acid...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Extracellular lipase; Endophytic yeast; Esterification; Submerged fermentation; Bioreactor.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822014000400047
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