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Vargas Júnior,Sergio Farias; Costa,Vitória Gasperin Guazzelli; Oliveira,Fernando Caetano de; Goularte,Karina Lemos; Haas,Cristina Sangoi; Gasperin,Bernardo Garziera; Lucia Júnior,Thomaz. |
Abstract: The objective of this work was to evaluate sperm cell motility after intrafollicular artificial insemination (IFAI) in vivo or after incubation in follicular fluid in vitro. In the in vivo experiment, IFAI was performed, followed by the recovery of follicular content 1 to 4 hours later, in order to assess sperm motility. In the in vitro experiment, spermatozoa from a pool of commercial frozen-thawed semen were evaluated for their kinetics after incubation for 1 or 3 hours, either pure (pool, control group) or in follicular fluid (FF). A low motility of sperm cells was observed in the FF samples, both in vitro and in vivo. In vitro, the main parameters negatively affected in the sperm cells incubated in FF, compared with the control, were: total... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Infertility; Insemination; Intrafollicular artificial insemination; Spermatozoa. |
Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-204X2018000700866 |
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Azevedo da Cunha Filho,Nilton; Oliveira,Plínio Aguiar de; Oliveira,Fernando Caetano de; Pappen,Felipe Geraldo; Aguiar,Cintia Lidiane Guidotti; Santos Junior,Alceu Gonçalves dos; Costa-da-Silva,André Luis; Leite,Fabio Pereira Leivas; Farias,Nara Amélia da Rosa. |
ABSTRACT: This study was conducted at a beef cattle breeding farm in the far southern region of Brazil. The birth of a calf with unilateral corneal opacity was immediately reported to the Laboratory of Parasitology, in the Universidade Federal de Pelotas, Rio Grande do Sul, Brazil; blood was collected from the cow and calf before colostrum intake. The umbilical cords from this calf and from six other healthy animals were collected. Serological examination, utilizing an indirect fluorescent antibody test, was done using a cut-off point of 1:100. Polymerase chain reaction (PCR) was also performed using the umbilical cord samples. Serological tests showed that the calf was positive for Neospora caninum at birth, with a titer of 1:1600; a titer of 1:3200 was... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Neospora caninum; Calves; Umbilical cord; Bovine. |
Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782017000700501 |
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