|
|
|
|
|
| Albuquerque,Pedro Paulo Feitosa de; Santos,André de Souza; Souza Neto,Orestes Luiz de; Kim,Pomy de Cássia Peixoto; Cavalcanti,Erika Fernanda Torres Samico Fernandes; Oliveira,Júnior Mário Baltazar de; Mota,Rinaldo Aparecido; Pinheiro Júnior,José Wilton. |
| Abstract The aim of this study was to detect the IS900 region of Mycobacterium avium subsp. paratuberculosis (MAP) in bovine milk samples using real-time polymerase chain reaction (qPCR) and conventional PCR, and to study the agreement between these tests. A total of 121 bovine milk samples were collected from herds considered positive for MAP, from the State of Pernambuco, Brazil. MAP DNA was detected in 20 samples (16.5%) using conventional PCR and in 34 samples (28.1%) using qPCR. MAP DNA was detected in all of the 6 animal farms studied. Moderate agreement was found between qPCR and conventional PCR results, where the sensitivity and specificity of conventional PCR in relation to qPCR were 50% and 96.6%, respectively. Thus, the IS900 region of MAP was... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Molecular biology; Diagnosis; Paratuberculosis; Milk. |
| Ano: 2017 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822017000100113 |
| |
|
|
| Santos,Sandra Batista dos; Melo,Renata Pimentel Bandeira de; Silva,Luana Thamires Rapôso da; Oliveira,Júnior Mário Baltazar de; Abad,Atzel Candido Acosta; Pinheiro Júnior,José Wilton; Mota,Rinaldo Aparecido. |
| ABSTRACT: The present study aimed to investigate contagious agalactia (CA) in flocks from Pernambuco State. The study involved 225 goats and 63 ewes; 288 milk samples and 100 vaginal swabs were collected in total. The PCR assays were carried out using specific primers to Mycoplasma agalactiae and the Mycoplasma mycoides cluster. Among the goat’s milk samples,12.0% (27/225) were positive for Mycoplasma agalactiae DNA, while 5.3% (12/225) contained the Mycoplasma mycoides cluster. Of the vaginal swabs taken from goats, 15.4% (12/78) were positive for Mycoplasma agalactiae DNA and 3.8% (3/78) contained the Mycoplasma mycoides cluster. In the case of ewes, 4.3% (1/23) of the milk samples contained Mycoplasma agalactiae DNA, and 7.5% (3/40) were positive for... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Contagious agalactia; Flocks; Diagnostic; Epidemiology; Risk factor.. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000400452 |
| |
|
|
|
