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Antimicrobial activity of an L-amino acid oxidase isolated from Bothrops leucurus snake venom J. Venom. Anim. Toxins incl. Trop. Dis.
Torres,AFC; Dantas,RT; Menezes,RRPPB; Toyama,MH; Filho,ED; Oliveira,MF; Nogueira,NAP; Oliveira,MR; Monteiro,HSA; Martins,AMC.
Some snake venom proteins present enzymatic activities, such as L-amino acid oxidase (LAAO). The aim of this paper was to investigate the effect of Bothrops leucurus total venom (BleuTV) and its fraction LAAO (BleuLAAO) on bacteria, yeast, and promastigote forms of Leishmania amazonensis and Leishmania chagasi, and epimastigote forms of Trypanosoma cruzi. BleuTV was isolated with a Protein Pack 5PW® (Waters Corporation, USA), and several fractions were obtained. BleuLAAO was purified to high molecular homogeneity, and its N-terminal amino acid sequence shared a high degree of amino acid conservation with other LAAOs. BleuTV inhibited Staphylococcus aureus growth in a dose-dependent manner, with a minimum inhibitory concentration (MIC) of 25 μg/mL, which...
Tipo: Info:eu-repo/semantics/article Palavras-chave: L-amino oxidase; Bothrops leucurus; Antimicrobial activity.
Ano: 2010 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1678-91992010000400012
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Meat Quality of Chicken Breast Subjected to Different Thawing Methods Rev. Bras. Ciênc. Avic.
Oliveira,MR; Gubert,G; Roman,SS; Kempka,AP; Prestes,RC.
Freezing is one of the methods to preserve and guarantee the quality of meat until it reaches the consumer. Even though freezing is classified as a mild form of preservation, it causes meat changes resulting from the formation of ice crystals that subsequently affect the tenderness and functionality of meat. The aim of this study was to evaluate the physicochemical and structural characteristics of chicken half breast submitted to fast freezing (-36 °C for 2 hours) and thawed by five different methods (under refrigeration, in a microwave, in a oven with air circulation, placed in cold water, or at room temperature). After thawing, the following parameters were evaluated: moisture content, drip loss (syneresis), water activity (aw), and shear force. Samples...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Texture; Hematoxylin-eosin; Fluid loss; Microwave.
Ano: 2015 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-635X2015000200165
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