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Immobilization of Lipases Produced by the Endophytic Fungus Cercospora kikuchii on Chitosan Microparticles BABT
Carneiro,Lara Aparecida Buffoni Campos; Costa-Silva,Tales Alexandre; Souza,Cláudia Regina Fernandes; Bachmann,Luciano; Oliveira,Wanderley Pereira; Said,Suraia.
This work studied the immobilization of Cercospora kikuchii lipases on chitosan microparticles by chemical attachment on chitosan acetate microparticles activated by glutaraldehyde (CAM) added before or after the enzyme and physical adsorption on highly deacetylated chitosan hydrochloride microparticles (CHM). Lipases covalently immobilized on pre-activated CAM showed better performance retaining 88.4% of the enzymatic activity, with 68.2% of immobilization efficiency (IE). The immobilized enzyme retained an activity of about 53.5 % after five reuses, using p-NPP as substrate. Physical adsorption of lipase onto highly deacetylated CHM showed 46.2 % of enzymatic activity and 28.6% of IE. This immobilized derivative did not lose activity up to 80 days of...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Enzyme immobilization; Chitosan; Cercospora kikuchii; Lipase.
Ano: 2014 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1516-89132014000400578
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Antioxidant activity and physical-chemical properties of spray and spouted bed dried extracts of Bauhinia forficata BJPS
Souza,Claudia Regina Fernandes; Georgetti,Sandra Regina; Salvador,Marcos José; Fonseca,Maria José Vieira; Oliveira,Wanderley Pereira.
Two distinct drying methods (spouted bed and spray drying) were used for production of dried extracts of Bauhinia forficata Link (Leguminosae, Caesalpinoideae). High-quality powder products in terms of physical and chemical properties were obtained. HPLC fingerprints revealed that the chromatographic profiles of flavonoid compounds present in the dried extract did not change significantly, due to drying. In general, the spouted bed drying caused a degradation of total flavonoids than was lower than that of the spray drying. Antioxidant properties of the dried extract, examined by their radical scavenging activity using 2,2-diphenyl-1-picrylhydrazyl radical (DPPH•) and inhibition of lipid peroxidation induced by Fe+2 assays (LPO), confirmed their...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bauhinia forficata/antioxidant activity; Bauhinia forficata/dried extract; Bauhinia forficata/properties; Flavonoids/qualitative analysis; Spouted bed/utilization; Spray drying/utilization.
Ano: 2009 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1984-82502009000200004
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Study of adsorption isotherms of green coconut pulp Ciênc. Tecnol. Aliment.
Lavoyer,Fábia Carolina Gonçalves; Gabas,Ana Lúcia; Oliveira,Wanderley Pereira; Telis-Romero,Javier.
Brazil is considered one of the largest producers and consumers of tropical fruits. Green coconut (Cocos nucifera L.) stands out not only for its production and consumption, but also for the high amount of waste produced by coconut water industry and in natura consumption. Therefore, there is a need for utilization of this by-product. This study aims to study the adsorption isotherms of green coconut pulp and determine its isosteric heat of sorption. The adsorption isotherms at temperatures of 30, 40, 50, 60, and 70 °C were analyzed, and they exhibit type III behavior, typical of sugar rich foods. The experimental results of equilibrium moisture content were correlated by models present in the literature. The Guggenheim, Anderson and De Boer (GAB) model...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Agro-industrial by-products; Green coconut; Thermodynamic properties; Sorption isotherms.
Ano: 2013 URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0101-20612013000100011
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