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| Silva,Rodrigo Otávio Silveira; Oliveira Junior,Carlos Augusto; Diniz,Amanda Nádia; Alves,Guilherme Guerra; Guedes,Roberto Maurício Carvalho; Vilela,Eduardo Garcia; Lobato,Francisco Carlos Faria. |
| The objective of this study was to evaluate antimicrobial susceptibility in Clostridium difficile strains isolated from animals and humans in Brazil. The 54 C. difficile strains used were isolated from stool samples from piglets (n=16), dogs (n=13), humans (n=13), foals (n=8) calves (n=2), an ocelot (n=1) and a maned wolf (n=1). Antimicrobial susceptibility was determined using the serial plate agar dilution method for penicillin, florfenicol, oxytetracycline, erythromycin, vancomycin, metronidazole and tylosin. The C. difficile strains assessed were susceptible to metronidazole and vancomycin. Florfenicol resistance was rarely observed; 52 (96.4%) strains were sensitive to this antimicrobial. Five (9.3%), five (9.3%), 14 (25.9%) and 20 (37.0%) strains... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Nosocomial diarrhea; Pseudomembranous colitis; Resistance. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000500013 |
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| Pinheiro,Carolina de Gouveia Mendes da Escóssia; Abrantes,Maria Rociene; Silva,Rodrigo Otávio Silveira; Oliveira Junior,Carlos Augusto; Lobato,Francisco Carlos Faria; Silva,Jean Berg Alves da. |
| ABSTRACT: The aim of this study was to evaluate the presence of microorganisms in honey produced by the stingless bee (SB) jandaíra (Melipona subnitida) from the semiarid region of Brazil. Thirty-five samples were analyzed and all of them were positive for mesophilic bacteria, coliforms at 45°C, fungi, and yeast. Staphylococcus spp. were identified in 85.7%, while Bacillus spp. were detected in 34.3% of honey samples. DNAs of Clostridium perfringens and C. botulinum were detected in 40% and 2.8% of the samples, respectively. Salmonella spp. and C. difficile were not detected. The present research revealed a great diversity of microorganisms in honey produced by jandaíra. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium botulinum; Clostridium perfringens; Stingless bee. |
| Ano: 2018 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782018000900450 |
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| Silva,Rodrigo Otávio Silveira; Oliveira Junior,Carlos Augusto; Guedes,Roberto Maurício Carvalho; Lobato,Francisco Carlos Faria. |
| Clostridium perfringens is a gram-positive, anaerobic, fermentative, spore-forming bacillus that may be found in the environment but is more commonly found as part of the microbiota of humans and animals. The bacterium is considered a common enteric pathogen; however, the pathogenesis and predisposing factors of the disease can differ among species. Therefore, specific studies are necessary for understanding the role of this pathogen, how to diagnose it and which control measures are applicable. The aim of this paper is to review the current knowledge regarding C. perfringens infections in pigs, horses and broiler chickens |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Diarrhea; Enteritis; Equine; Swine; Poultry. |
| Ano: 2015 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782015000601027 |
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| Silva,Rodrigo Otávio Silveira; Guedes,Roberto Maurício Carvalho; Gabardo,Michelle de Paula; Oliveira Junior,Carlos Augusto; Salvarani,Felipe Masiero; Pires,Prhiscylla Sadanã; Alves,Guilherme Guerra; Lobato,Francisco Carlos Faria. |
| O objetivo do presente trabalho foi padronizar um modelo de infecção por Clostridium difficile (ICD) em hamsters sírios (Mesocricetus auratus). Para seleção dos isolados capazes de causar letalidade, cinco animais por grupo receberam uma dose de clindamicina (30mg kg-1) por gavagem. Após 48 horas, administraram-se 107 unidades formadoras de colônia (UFC), por animal, de quatro diferentes isolados toxigênicos de C. difficile. Selecinou-se um dos isolados capazes de causar diarreia e letalidade e administrou-se 4x102; 4x104; 4x106; 4x108UFC por animal, novamente com cinco hamsters por grupo. Em todas as diluições testadas, foi possível observar a ocorrência de diarreia e morte. A maior concentração testada (4x108UFC por animal) causou óbito de 100% dos... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Clostridium difficile; Diarreia nosocomial; Modelo animal; Infecção hospitalar. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000801415 |
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| Silva,Rodrigo Otávio Silveira; Ferreira Junior,Francisco Carlos; Marques,Marcus Vinícius Romero; Oliveira Junior,Carlos Augusto; Martins,Nelson Rodrigo da Silva; Lobato,Francisco Carlos Faria. |
| The aim of this study was to isolate, genotype and evaluate the antimicrobial susceptibility of Clostridium perfringens found in species Tinamidae, Cracidae and Ramphastidae in Brazil. C. perfringens was isolated in 13 (5%) out of 260 swab samples and five (8.3%) out of 60 stool samples. All strains were classified as C. perfringens type A, and nine (50%) were positive for the beta-2 toxin-encoding gene. No strains were positive for the necrotic enteritis toxin B-like (NetB)-encoding gene. All isolates were susceptible to penicillin, metronidazole and vancomycin, whereas four (22.2%), five (27.8%) and 13 (72.2%) strains were considered resistant to erythromycin, oxytetracycline and lincomycin, respectively. |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Necrotic enteritis; Avian; Toucan. |
| Ano: 2014 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0103-84782014000300016 |
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