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Registros recuperados: 37
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Phenotypic characterization of the marine pathogen <i>Photobacterium damselae</i> subsp. <i>piscicida</i> OMA
Thyssen, A.; Grisez, L.; van Houdt, R.; Ollevier, F.P..
The taxonomic position of <i>Photobacterium damselae</i> subsp. <i>piscicida</i>, the causative agent of fish pasteurellosis, is controversial as this organism has also been described as '<i>Pasteurella piscicida</i>'. To clarify the taxonomic position of the pathogen, a total of 113 <i>P. damselae</i> subsp. <i>piscicida</i> strains and 20 <i>P. damselae</i> subsp. <i>damselae</i> strains, isolated from different geographical areas and from the main affected fish species, were analysed using 129 morphological and biochemical tests, including the commercial API 20E and API CH50 test systems. For comparison, the type strains of other <i>Photobacterium</i> species...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Numerical taxonomy.
Ano: 1998 URL: http://www.vliz.be/imisdocs/publications/238535.pdf
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The composition of the fish and crustacean community of the Zeeschelde estuary (Belgium) OMA
Maes, J.; Taillieu, A.; Van Damme, P.A.; Ollevier, F.P..
Fykes were used to monitor the fish and crustacean fauna of the mudflats of the Zeeschelde between April 1995 and December 1996. Overall 38 species were recorded; the number of species was greater in the brackish water part than in the fresh water part probably due to reduced oxygen concentrations in the upper reaches of the river. In terms of wet weight, just 6 species accounted for more than 96% of the total annual catch. These species are shore crab, eel, flounder, common shrimp, sole and bass. Clear seasonal patterns in the community structure were not identified. It is argued that the selectivity of the fishing gear is mainly responsible for the dominance of a few species and the lack of a temporal structure.
Tipo: Info:eu-repo/semantics/article
Ano: 1997 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=1409
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Hatching, survival, activity and penetration efficiency of second-stage larvae of <i>Anguillicola crassus</i> (Nematoda) OMA
Thomas, K.M.; Ollevier, F.P..
Hatching of eggs of <i>Anguillicola crassus</i> was studied at temperatures between 5 and 30°C. Hatching occurred between 10 and 30°C and the incubation time was inversely related to temperature. Survival, spontaneous and excitatory rate of activity of 2nd-stage larvae of <i>A. crassus</i> were also affected by temperature. The percentage of larvae eaten by <i>Paracyclops fimbriatus</i> was not always influenced by the activity of the larvae. Penetration efficiency into the haemocoel of the intermediate host was highest for freshly hatched larvae and declined gradually with age. Completely non-active larvae were still able to penetrate, although penetration efficiency was lower.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Anguillicola crassus.
Ano: 1993 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2788
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Use of computer assisted sperm analysis (CASA) for monitoring the effects of pollution on sperm quality of fish: application to the effects of heavy metals OMA
Kime, D.E.; Ebrahimi, M.; Nysten, K.; Roelants, I.; Rurangwa, E.; Moore, H.D.M.; Ollevier, F.P..
Computer assisted sperm analysis (CASA) has been used to analyse the effects of cadmium and zinc on the motility of fish sperm treated either for 24h after partial dilution in an extender, or during the final dilution step on the microscope stage during video recording. The progressive motility of catfish sperm, which was not affected by storage for 24h, was decreased after exposure to 100 ppm cadmium or 2000 ppm zinc in extender for 24h. Only 1000 ppm cadmium or 2000 ppm zinc had any effect on motility when added to sperm at the final dilution stage. These results suggest that concentrations of cadmium which have been found to be present in fish gonads as a result of bioaccumulation from the water or diet may decrease the motility of sperm during...
Tipo: Info:eu-repo/semantics/article
Ano: 1996 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2993
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An improved method for artificial infection of the European eel, <i>Anguilla anguilla</i>, with <i>Anguillicola crassus</i> (Nematoda, Dracunculoidea) OMA
De Charleroy, D.; Cannaerts, V.; Augustijn, H.; Grisez, L.; Boon, J.; Ollevier, F.P..
A standardized method was developed for artificially infecting the European eel with <i>Anguillicola crassus</i>, a parasitical swim bladder nematode. For this purpose, an intermediate host, <i>Paracyclops fimbriatus</i>, and a paratenic host, <i>Cyprinus carpio</i>, were used. Parasite prevalence in the artificially infected eels was very high, although there was a large variation in the number of worms per eel. Compared to other methods of artificial infection described in the literature, this method appears to be more efficient ad reliable; there were also more infectious larvae that were recovered and the mortality rate of eels under treatment was lower.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Hosts Infestation Invertebrate larvae Methodology Mortality Swim bladder Anguilla anguilla (Linnaeus; 1758) Anguillicola crassus Copepoda [copepods] Cyprinus carpio Nematoda [Nematodes] Paracyclops fimbriatus (Fischer; 1853).
Ano: 1990 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3073
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The use of API 20E for the identification of <i>Vibrio anguillarum</i> and <i>V. ordalii</i> OMA
Grisez, L.; Ceusters, R.; Ollevier, F.P..
Sixty-eight strains of <i>Vibrio anguillarum</i>, five of <i>V. ordalii</i> and the type strains of <i>V. alginolyticus, V. carchariae, V. damsela</i> and <i>V. parahaemolyticus</i> were compared using the API 20E gallery. Within the <i>V. anguillarum</i> strains, distinct groups could be separated mainly on the basis of their reaction on indole production and the fermentation of amygdalin and arabinose. <i>Vibrio ordalii</i>, the former <i>V. anguillarum</i> biotope 2, could easily be separated from <i>V. anguillarum</i> and from the other fish pathogenic <i>Vibrio</i> spp.
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial diseases; Disease control; Disease detection; Fish diseases; Identification; Vibrio carchariae; Vibrio ordalii; Vibrio alginolyticus; Vibrio anguillarum; Vibrio damsela; Vibrio parahaemolyticus.
Ano: 1991 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2859
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The effect of supplemental ascorbic acid in enriched live food for <i>Clarias gariepinus</i> larvae at startfeeding OMA
Merchie, G.; Lavens, P.; Verreth, J.; Ollevier, F.P.; Nelis, H.; De Leenheer, A.P.; Storch, V.; Sorgeloos, P..
The effect of three dietary ascorbic acid (AA) concentrations, each applied via two feed types, on production characteristics and physiological condition of African catfish (<i>Clarius gariepinus</i>) larvae has been assessed in two 10-day culture trials. Three treatments received only <i>Artemia</i> nauplii enriched with an experimental emulsion containing 0, 10 or 20% ascorbyl palmitate (AP) and yielding 530, 1200 and 1600 µg AA g<sup>-1</sup> DW <i>Artemia</i> , respectively; the other three treatments were fed the same <i>Artemia</i> diets which were partially substituted by an artificial diet containing no vitamin C (ratio 20:80). No differences in survival could be observed: however, from...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Fish larvae; Food organisms; Vitamin C; Clarias gariepinus.
Ano: 1997 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2963
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Search for beneficial bacterial strains for turbot (<i>Scophthalmus maximus</i> L.) larviculture OMA
Huys, L.; Dhert, P.; Robles, R.; Ollevier, F.P.; Sorgeloos, P.; Swings, J..
The aerobic bacterial flora in the gut of turbot larvae and their influence on larval survival was examined. Two turbot experiments were run with six replicates each time. Large variation, from 0% up to 44%, was observed in the survival percentage of turbot larvae. There was no correlation between the number of bacteria present in the gut of turbot larvae and the larval survival rate. During both experiments, all replicates followed nearly the same rate of bacterial development in the gut of turbot larvae going from circa 10<sup>2</sup> CFU larva<sup>-1</sup> just before first feeding at day 3 post hatch to 10<sup>5</sup> CFU larva<sup>-1</sup> at day 9 post hatch.In total, 127 bacterial isolates from 12...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Aerobic bacteria; Aquaculture; Commensals; Digestive system; Fish culture; Fish larvae; Gastrointestinal tract; Microorganisms; Rearing; Scophthalmus maximus; Survival; Bacteria; Scophthalmus maximus; Vibrio mediterranei.
Ano: 2001 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=5720
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The life cycle of <i>Anguillicola crassus</i> OMA
De Charleroy, D.; Grisez, L.; Thomas, K.; Belpaire, C.; Ollevier, F.P..
For some years now the parasitic swim bladder nematode <i>Anguillicola crassus</i> of the European eel <i>Anguilla anguilla</i> L., has been reported from several European countries. The entire life history of this parasite has recently been elucidated in our laboratory. Young larvae leave the swim bladder of the host via the pneumatic duct and reach the water through the digestive tract. They are ingested by small copepods (Cyclopoida), which act as intermediate hosts. Larvae remain in the hemocoel until the copepods are eaten by the final host, the European eel. Larvae penetrate through the intestinal wall and reach the swim bladder where they develop into adults. When infected copepods are eaten by other small fish, such as carp...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Biological vectors; Disease transmission; Fish diseases; Food chains; Freshwater fish; Hosts; Life cycle; Parasites; Parasitic diseases; Predation; Predators; Swim bladder; Anguillicola crassus.
Ano: 1990 URL: http://www.vliz.be/imisdocs/publications/3074.pdf
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Extended serotyping scheme for <i>Vibrio anguillarum</i> with the definition and characterization of seven provisional O-serogroups OMA
Pedersen, K.; Grisez, L.; van Houdt, R.; Tiainen, T.; Ollevier, F.P.; Larsen, J.L..
The present paper summarizes the serotyping scheme of the fish pathogenic bacterium <i>Vibrio anguillarum</i> and defines seven additional O-serogroups. Strains, collected in our laboratories that were nontypable with antisera against the previously defined 16 O-serotypes, were used for generating new antisera and were characterized further by means of LPS profiles, Western blots, and serological reactions. On the basis of the results, it is suggested that the seven new O-serogroups are to be included in the existing serotyping system as serotypes O17-O<sub>2</sub>3. However, the existence of further <i>V. anguillarum</i> strains that were not typable with any of the 23 O-antisera suggested the existence of additional...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Bacterial diseases; Fish diseases; Pathogenic bacteria; Pathogens; Serological studies; Serotypes; Strains; Vibrio anguillarum.
Ano: 1999 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=9525
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The population dynamics of <i>Lernaeocera lusci</i> and <i>L. branchialis</i> on intermediate hosts OMA
Van Damme, P.A.; Ollevier, F.P..
The metapopulation dynamics of <i>Lernaeocera lusci</i> and <i>L. branchialis</i> on sole <i>Solea solea</i> and flounder <i>Pleuronectes flesus</i> were studied in the Dutch coastal area. Both fish species harboured large numbers of parasites when they arrived in the coastal area in the spring. Between April and June all parasites detached from the intermediate hosts and infected the definitive hosts (0+ whiting <i>Merlangius merlangus</i> for <i>L. branchialis</i>, and possibly sand goby <i>Pomatoschistus minutus</i> for <i>L. lusci</i>). Thereafter, flounder remained almost parasite-free until autumn. This suggests that <i>L. branchialis</i> has...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Parasites Population dynamics Lernaeocera branchialis Lernaeocera lusci ANE; North Sea.
Ano: 1996 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3143
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Morphological and morphometric study of crustacean parasites within the genus <i>Lernaeocera</i> OMA
Van Damme, P.A.; Ollevier, F.P..
Two species of <i>Lernaeocera</i> are present in the southeastern North Sea. <i>Lernaeocera lusci</i> infects bib <i>Trisopterus luscus</i>, dragonet <i>Callionymus lyra</i> and sand goby <i>Pomatoschistus minutus. L. minuta</i> is a junior synonym of <i>L. lusci.</i>. The second valid species, <i>L. branchialis</i>, infects whiting <i>Merlangius merlangus</i>. The two species can be morphologically separated by the antennary processes, which are present in <i>L. lusci</i> and absent in <i>L. branchialis</i>. Discriminant functions allow complete separation between <i>L. lusci</i> and <i>L. branchialis</i>. There...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Lernaeocera Blainville; 1822.
Ano: 1995 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3130
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Experimentally induced infections of European eel <i>Anguilla anguilla</i> with <i>Anguillicola crassus</i> (Nematoda, Dracunculoidea) and subsequent migration of larvae OMA
Haenen, O.L.M.; Grisez, L.; De Charleroy, D.; Belpaire, C.; Ollevier, F.P..
Migration patterns of third-stage <i>Anguillicola crassus</i> larvae, and pathogenesis of the lesions induced by third-stage larvae, was investigated in European eel <i>Anguilla anguilla</i> L. Young elvers (1g) were fed infected <i>Paracyclops fimbriatus</i> (Copepoda). Eel samples were collected and examined histologically at varying intervals during 6 mo post-infection period. Third-stage larvae (L-III) migrated directly through the intestinal wall and body cavity to the swimbladder within 17h post-infection. L-IV larvae were detected 3 mo post-infection, and immature adults were detected within 4 mo post-infection. The parasites occasionally showed aberrant migration paths. Pathological effects caused by the parasite...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Larvae Life cycle Pathology Anguilla anguilla (Linnaeus; 1758) Anguillicola crassus.
Ano: 1989 URL: http://www.vliz.be/imisdocs/publications/3029.pdf
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Application of AFLP for taxonomic and epidemiological studies of <i>Photobacterium damselae</i> subsp. <i>piscicida</i> OMA
Thyssen, A.; Van Eygen, S.; Hauben, L.; Goris, J.; Swings, J.; Ollevier, F.P..
A collection of 106 <i>Photobacterium damselae</i> subsp. <i>piscicida</i> strains and 19 <i>Photobacterium damselae</i> subsp. <i>damselae</i> strains, including reference and type strains, were genetically characterized using AFLP. The total genomic DNA of each bacterial strain was digested using restriction endonucleases <i>Hind</i>III and <i>Taq</i>I. Using numerical analysis, six clusters were recognized. The largest cluster (n=106) contained the majority of the strains tested and consisted exclusively of <i>Photobacterium damselae</i> subsp. <i>piscicida</i>. The <i>Photobacterium damselae</i> subsp. <i>damselae</i> strains fell...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Epidemiology; Taxonomy.
Ano: 2000 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=9527
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Live food mediated vitamin C transfer to <i>Dicentrarchus labrax</i>and <i>Clarias gariepinus</i> OMA
Merchie, G.; Lavens, P.; Dhert, Ph.; Pector, R.; Mai Soni, A.F.; Nelis, H.; Ollevier, F.P.; De Leenheer, A.P.; Sorgeloos, P..
Live food enrichment techniques, using formulated diets and emulsions for improving the nutritional quality of <i>Brachionus</i> and <i>Artemia</i> , were studied as a tool for transferring ascorbic acid (AA) to fish larvae. <i>Artemia</i> nauplii enriched for 24h with an experimental emulsion containing 20% HUFA and 0%, 10% and 20% ascorbyl palmitate (AP) were administered to catfish larvae in a 20-day feeding trial. Survival was not affected by the dietary AA, but from day 7 onwards a significantly positive effect of supplemental AA on growth was demonstrated. At the end of the experiment the 20% AP group weighed 30% more than the control (0% AP), i.e. 9.5 and 6.3mg DW, respectively. Evaluation of the physiological...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Clarias gariepinus Dicentrarchus labrax (Linnaeus; 1758) [Bass].
Ano: 1995 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2581
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Endoparasitic helminths of the whitespotted rabbitfish (<i>Siganus sutor</i> (Valenciennes, 1835)) of the Kenyan coast: distribution within the host population and microhabitat use OMA
Geets, A.; Ollevier, F.P..
The parasitic fauna of the alimentary tract of adult whitespotted rabbitfish, <i>Siganus sutor</i>, sampled in December 1990 at the Kenyan coast, was investigated. Five endoparasites were found: the digenean trematodes <i>Opisthogonoporoides hanumanthai, Gyliauchen papillatus</i> and <i>Hexangium sigani</i>, the acanthocephalan <i>Sclerocollum rubrimaris</i> and the nematode <i>Procammalanus elatensis</i>. No uninfected fish, nor single species infections occurred. Parasite population data showed very high prevalences for all endoparasites, ranging from 68.18% to 100%. <i>G. papillatus</i> occurred with the highest mean intensity, 201.68 ± 12.54 parasites per infected fish. The...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Siganus sutor [shoemaker spinefoot] ISW; Kenyan Coast.
Ano: 1996 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2966
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The population dynamics of the parasitic copepode <i>Lernaeocera lusci</i> (Bassett-Smith, 1896) on its definitive host OMA
Van Damme, P.A.; Hamerlynck, O.; Ollevier, F.P..
The mesoparasitic copepod <i>Lernaeocera lusci</i> (Bassett-Smith, 1896) was recovered from first-year bib (<i>Trisopterus luscus</i> L.) in the Voordelta (Southern Bight of the North Sea) from May until December 1989. Analysis of the seasonal abundance and of the population structure showed that transmission of infective stages to bib mainly occurred from June to September. From September to December the overall prevalence fluctuated around 70%. Maximum parasite population size (47/10<sup>4</sup>m²) and the highest total egg number were recorded in September and October, respectively. It was found that total parasite mortality was significantly influenced by mortality of hosts carrying parasites. Natural mortality...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Parasites Population dynamics Lernaeocera lusci ANE; North Sea.
Ano: 1996 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=3115
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A comparative study on the use of different preparations of decapsulated <i>Artemia</i> cysts as food for rearing African catfish (<i>Clarias gariepinus</i>) larvae OMA
Pector, R.; Tackaert, W.; Abelin, P.; Ollevier, F.P.; Sorgeloos, P..
African catfish (<i>Clarias gariepinus</i>) larvae were fed on five preparations of decapsulated Great Salt Lake <i>Artemia</i> cysts: 1) dried (35°C, 6 h) and UV-irradiated cysts; 2) heated cysts (80°C, 10 min); 3) brine dehydrated and UV irradiated cysts; 4) micro-bound diet 1(with intact decapsulated cysts used in diet preparation 1); and 5) micro-bound diet 2 (with crushed and shifted decapsulated diet used in diet preparation 1). The larvae were fed from the first day after yolk-sac resorption (fourth day after hatching). After a 14-d rearing period, larvae fed on dried or heated decapsulated cysts or micro-bound diet 1. Feeding micro-bound diet 2 resulted in a significantly lower average gain in weight as compared to the other...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Artemia; Clarias gariepinus.
Ano: 1994 URL: http://www.vliz.be/nl/open-marien-archief?module=ref&refid=2660
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Mode of infection and spread of <i>Vibrio anguillarum</i> in turbot <i>Scophthalmus maximus</i> larvae after oral challenge through life feed OMA
Grisez, L.; Chair, M.; Sorgeloos, P.; Ollevier, F.P..
The infection route of the marine fish pathogen <i>Vibrio anguillarum</i> was studied after oral challenge of the juvenile turbot <i>Scophthalmus maximus</i> L. through a life feed. <i>Artemia</i> nauplii were incubated in a suspension of <i>V. anguillarum</i> cells, and subsequently fed twice to the fish. All challenged fish died within 4d after the first challenge, while no mortality occurred in the non-challenged controls. The results of an immunohistochemical examination of the sectioned fish samples clearly demonstrated that <i>V. anguillarum</i> cells were ingested by the <i>Artemia</i> and that the latter were ingested by the fish. Bacteria were released from the...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Scophthalmus maximus (Linneaus; 1758) Vibrio anguillarum.
Ano: 1996 URL: http://www.vliz.be/imisdocs/publications/299074.pdf
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Population biology of <i>Anguillicola crassus</i> in the final host <i>Anguilla anguilla</i> OMA
Thomas, K.; Ollevier, F.P..
Eels were sampled twice a month (March 1990 though March 1991) at the Kolenhaven (Albertcanal, Genk, Belgium) and examined for <i>Anguillicola crassus</i> nematodes in the swimbladder. Averaged over all samples, 90.2% of the 345 eels were infected with a mean intensity (averaged for all stages) of 17 nematodes. Thirty-one percent of all nematodes, which were adult worms or their remnants, were present in the swimbladder lumen, while the other 69% which consisted of the third or fourth larval stage and preadults were found in the swimbladder wall. No clear seasonal fluctuations in parasite prevalence or mean intensity were observed. Both L<sub>3</sub>-larvae and mature adult worms were present throughout the year. Eggs or...
Tipo: Info:eu-repo/semantics/article Palavras-chave: Abundance Endoparasites Life history Parasitic diseases Population density Seasonality Statistical analysis Swim bladder Anguilla anguilla (Linnaeus; 1758) Anguillicola crassus Belgium.
Ano: 1992 URL: http://www.vliz.be/imisdocs/publications/299071.pdf
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