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| Alfenas,Poliane F.; Braz,Antonio Sérgio K.; Torres,Leonardo B.; Santana,Enilton N.; Nascimento,Ana Verônica S. do; Carvalho,Murilo G. de; Otoni,Wagner C.; Zerbini,F. Murilo. |
| Sixteen transgenic yellow passionfruit (Passiflora spp.) plants (R0) were obtained which express a non-translatable transgenic RNA corresponding to the 3' region of the NIb gene and the 5' region of the CP gene, derived from the genome of a Brazilian isolate of Cowpea aphid-borne mosaic virus (CABMV). The transgenic plants were propagated by stem cuttings and challenged by sap inoculation with isolates CABMV-MG1 and CABMV-PE1. One transgenic plant (TE5-10) was resistant to the isolate CABMV-MG1, but susceptible to CABMV-PE1. The remaining transgenic plants developed systemic symptoms, equal to non-transformed plants, when inoculated with either isolate. The absence of virus in TE5-10 plants was confirmed by indirect ELISA. Transcription analysis of the... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Passionfruit woodiness virus; PWV; Potyvirus; Resistance. |
| Ano: 2005 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S0100-41582005000100006 |
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| Festucci-Buselli,Reginaldo A.; Contim,Luis A. S.; Barbosa,Luiz Cláudio A.; Stuart,Jeff J.; Vieira,Roberto F.; Otoni,Wagner C.. |
| The ecdysteroid 20-hydroxyecdysone (20E) is a steroid hormone found in insects and few plants. To analyze its temporal and spatial distribution in Pfaffia glomerata (Amaranthaceae), we selected among 71 accessions, the accession 13 which showed the highest root 20E total quantity (1.48 g/plant root). The 20E was constantly detected in flowers, leaves, stems, and roots, but its percentage was variable throughout its development. The highest 20E percentage was found in flowers (0.82%), roots (0.66%), leaves (0.60%), and stems (0.24%). While stems showed the least variable 20E percentage (0.13% - 0.24%), followed by roots (0.42% - 0.66%), and flowers (0.47% - 0.82%). Leaves showed the greatest 20E percentage variation (0.21% - 0.60%). The stem and leaves 20E... |
| Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Pfaffia glomerata (Spreng.) Pedersen; Amaranthaceae; Brazilian ginseng; Phytochemical analysis; Terpenes; Phytoecdysteroid; 20-hydroxyecdysone. |
| Ano: 2008 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1677-04202008000400006 |
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