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Medina,Gustavo; Otth,Carola; Otth,Laura; Fernández,Heriberto; Muñoz,Celeste; Cruz,María; Zaror,Ángela; Henriquez,Ruby; Arce,Maria; Wilson,Myra. |
The cassette chromosome mec (SCCmec) present in methicillin-resistant Staphylococcus aureus (MRSA) has two essential components, the ccr gene complex and the mec gene complex. Additionally, SCCmec has non-essential components called J regions which are used for MRSA subtyping. This study was performed to determine subtypes MRSA strains carrying SCCmec type I based on polymorphism of regions located downstream of the mecA gene. A total of 98 MRSA strains carrying SCCmec type I isolated from patients hospitalized at the County Hospital of Valdivia (Chile) between May 2007 and May 2008, were analyzed by multiplex PCR designed to amplify the mecA gene and 7 DNA hypervariable regions located around the mecA gene. MRSA strains were classified into seventeen... |
Tipo: Info:eu-repo/semantics/article |
Palavras-chave: Subtypified MRSA; Polymorphism MRSA; SCCmec MRSA. |
Ano: 2012 |
URL: http://www.scielo.br/scielo.php?script=sci_arttext&pid=S1517-83822012000300006 |
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